How to check pH of PCR mastermix?

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Sven Reister

Why do you want to measure it? If you do not know the buffer system this will be difficult as pH is temperature dependent and therefore pH at 25°C and at 60°C differ.

Best Sven

Paul Rutland

A small drop on to pH indicator paper will give you the pH at room temperature

Oghenevowhero Beatrice Ereziwosa

To check the pH of a PCR master mix:

Obtain pH indicator strips or a pH meter.

Dip the strip into the master mix or use the meter to measure the pH directly.

Match the strip color or read the pH value from the meter against the pH scale to determine the acidity/alkalinity.

Ensure the pH is within the optimal range for PCR (typically 8.0 to 8.5). Adjust if necessary using small amounts of acidic or basic solutions.