I am trying to transfer a group of bacterial strains but they loose the plasmid in the down stream processes. Iam wondering if i can be to transform them in a way that the gene of interest gets incorporated in the chromosome.
Why is your plasmid getting lost? Have you not put selection pressure (resistance to antibiotics)?
To force the gene of interest to integrate the chromosome, you must carry out homologous recombination (single or double) and your plasmid must be non-replicative to force this recombination.