I am analysing heavy metals in soil samples via ICP-OES and now I need to make calibration curves for heavy metals and for that I have to do serial dilutions from already prepared stock sloution In order to get a straight line. Plus shall I only take volumes or shall I measure them on weighing balance?
Please do not use serial dilutions. Please consider the reasons for that suggestion, and consult reference texts. You may take volumes but I suggest that you also measure the SG of the stock standard and measure the masses of the pipetted volumes as a check. You will probably end up using the masses in the calibration.
The international standard for ICP is to measure only between standards, i.e., not below the lowest standard. Look at the residuals of the calibration to determine how well it fits. Have an independent standard to check the calibration and a suitable reference material to check the 'recovery'.
Be aware that matrix matching is necessary for accurate results
- Badges
- Science topic
- Similar topics
- Biological Science
- Biochemistry
- Biochemical Techniques
- Analytical Chemistry
- pH