How can I calculate Tm of DNA secondary structure?

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Manendra Babu

Tm = 4(G+C) +2(A+T)

Denis V Antonets

http://en.wikipedia.org/wiki/Nucleic_acid_thermodynamics

SantaLucia J., Hicks D. The thermodynamics of DNA structural motifs. Annu

Rev Biophys Biomol Struct. 2004;33:415-40. (http://www.annualreviews.org/doi/abs/10.1146/annurev.biophys.32.110601.141800)

Mikolaj Adamek

Try Oligo Analyzer http://eu.idtdna.com/analyzer/Applications/OligoAnalyzer/