usually we use -mother solution of 30 micromol TBA (as TEP) and use from 0.3 to 10 nmol,TBA per well of 250 microlitters
Dear Majid,
in both raw and cooked meat, we assess lipid oxidation by measuring 2-thiobarbituric acid reactive substances (TBARS) according to the method described by Siu and Draper (1978).
Reference:
Siu, G.M., & Draper, H.H. (1978). A survey of the malonaldehyde content of retail meats and fish.Journal of Food Science, 43, 1147–1149
Dear Majid Aminzare ,
Follow this publication: Botsoglou, N. A., Fletouris, D. J., Papageorgiou, G. E., Vassilopoulos, V. N., Mantis, A. J., & Trakatellis, A. G. (1994). Rapid, sensitive, and specific thiobarbituric acid method for measuring lipid peroxidation in animal tissue, food, and feedstuff samples. Journal of Agricultural and Food Chemistry, 42(9), 1931-1937.
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