How can I calculate the r2 value for linkage disequilibrium?

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How to prevent getting fragmented villi structure in HE staining of small intestine sections?

I am trying to perform HE staining of small intestine. I used OCT solution to make sections. After HE staining when I look my slides under microscope, I do not get intact villi. In much of the...

01 February 2020 5,960 3 View

Is there any method to measure serum protein besides ELISA?

We are currently working on a cytosolic protein; however, we think that this protein also has a secreted form and can be detected in the bloodstream. As there is no ELISA kit available for this...

01 February 2020 468 2 View

Can stimulatory signal induce binding of two overexpressed proteins in HEK293T cells?

I want to study whether two proteins: Myc-tagged protein A and Flag-tagged protein B, interacts with each other or not. For that I co-expressed these proteins in HEK293T cells, and later (after 48...

01 February 2019 1,927 4 View

Can HEK293T cells be used to study non-direct interaction between exogenously induced mouse proteins?

I want to determine whether protein A and protein B interacts with each other by co-immunoprecipitation analysis. For that I prepared Myc-tagged protein A and Flag-tagged protein B plasmid DNAs. I...

01 February 2019 994 4 View

Do immortalized BMDM can be purchased commercially?

I am just wondering whether different labs prepare immortalized bone marrow derived macrophages (BMDM) in their labs. I have experience of preparing primary BMDM from mice femur, but have no idea...

31 December 2018 8,956 2 View

Endogenous IP vs expressed IP?

I have been working on two proteins. And I think that these two proteins interact with each other. To prove that, I analyzed there association by endogenous IP, but unfortunately I could not get...

11 December 2018 9,770 4 View

Why books contain only references but no citations?

Articles published in journals contains both reference and citation, while books contain only reference. Does anybody have idea about it?

09 October 2018 971 0 View

What is the function of EDTA in binding buffer for EMSA?

I just started to do EMSA to check interaction of a protein and DNA. When I checked the binding buffer used for EMSA, I found that EDTA is also used. I know that EDTA is a chelating agent, and...

04 May 2018 2,719 1 View

How can I increase the yield of primary microglia harvest?

I have been recently trying to isolate primary microglia from mice pups brain (P0-P1). To prepare mixed microglia culture, I used 3 brains for a 75 cm2 flask. After around 10-12 days, astrocyte...

03 April 2018 3,801 12 View

How can we increase Lenti-virus titre?

Currently, I am doing knockdown of certain genes by shRNA technique. We use lenti-virus for this process. The problem is that we are getting low titre. The experimental procedure is: Day 1: Seed 7...

04 May 2017 2,452 6 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

Which Scopus Journal provides the most affordable fees?

"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....

10 August 2024 8,621 1 View

Seeking Advice on Viability and Execution of Undergraduate Thesis Topic?

Hello everyone, I am currently developing a thesis proposal and would appreciate your input on its viability and how to effectively carry it out. My proposed topic is: "Does the perceived threat...

10 August 2024 8,992 0 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

Who will be moral responsible for the death of thousands of people in the event of an earthquake?

Who will bear moral responsibility for the deaths of thousands of people in the event of an earthquake? Weeks and months remain before the onset of strong earthquakes that bring death to...

08 August 2024 6,134 12 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View