I´m making adsorption experiments with peptides onto biomaterials, but I have no idea of calculating the N and K parameters and its error from the plotted data. If anyone can help me I´d be very pleased. Thank you!
You can read one of my paper which may provide you some information.
Synthesis and characterization of cotton-made activated carbon fiber and its adsorption of methylene blue in water treatment
KL Chiu, DHL Ng - Biomass and Bioenergy, 2012
The error can be calculated from the standard derivation of measured value of adsorption capacity qe with the value of qe obtained with the model qe. Be careful not to directly use the R2 value get in the fitted curve calculated with equation (7).
I finally got the error of the N and K parameters by making a regression analysis of the data with Statgraphics Centurion; a statistical analysis software. With it, you get the slope and the intercept values, as well as their associated standard deviation. Just converting the standard deviation values as you have to do for this model to get the N and K values, you will get all the information that you need.
Find attached a screenshot of the result of the regression analysis for further clarification.