Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

Is there any good online workshop on advanced DEA models?

OR ANY study material with step by step analysis & its interpretation using the software?

01 January 2021 993 1 View

How to convert RGB image of a stable diffusion flame captured from a mobile phone camera to thermal image?

I need the temperature profile (a quantitative dataset) of the flame under study. Is there any image processing tool a available which I can utilize for my study?

18 December 2020 2,707 2 View

Why gel electrophoresis is taking too much time to run ?

I am working with TBE buffer and prepared 1% agarose gel. Previously it was taking around one hour to run but now two and half hours. I have increased the voltage from 90 to 110 but this also helped.

08 December 2020 7,416 5 View

What are the recent research topics in field of Soft Robotics based on AI and ML?

I am working in the field of practical robotics since last five years and got acquainted with AI and ML recently. I know Python and learning AI and ML using python from different sources. I want...

24 October 2020 793 6 View

Future of cadaveric dissection in anatomy teaching after COVID Pandemic?

Cadaver dissection are the best adopted and accepted teaching tool in anatomy teaching from the accident time till date. Does the dissecting room still have a place in educating our under- and...

02 August 2020 2,948 17 View

Can you please describe the below microstructure?

The below micro structure of Spindle which failed during rolling process hot rolled plates , this is having 0.39%carbon, can anyone please explain the micro structure.

12 May 2020 2,116 1 View

Can Solar Energy take part in fighting against COVID-19?

Can "Solar-UV-cellular-damage" be an effective method to disinfect water bodies against COVID-19 in countries with a hot climate?

10 May 2020 2,556 12 View

While performing energy minimization step in GROMACS I am getteing following error. Can anyone help with this minimization problem?

gmx mdrun -v -deffnm em Back Off! I just backed up em.log to ./em.log. Running on 1 node with total 8 cores, 16 logical cores Hardware detected: CPU info: Vendor: GenuineIntel Brand:...

11 March 2020 2,902 1 View

What is iodine number in terms of activated carbon? What are the effect of iodine number on activate carbon porosity ?

11 March 2020 5,239 3 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View