How can i calculate fold change from ct values using delta delta ct method.?

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Michael Förster

Hi Vikas,

please consider this landmark paper from Schmittgen and Livak. It best describes the delta delta ct method.

Here is the link:

https://www.nature.com/articles/nprot.2008.73

All the best & kind regards,

Michael

Antonio di stasi

Hello,

excellent advise in the previous posts. Here is a simple excel datasheet with formulas. Hope it is helpful.

Regards,

Online resources from IRIC in regard to explanation of abbreviations and methods.

Standard curve method datasheet and fold induction.

Smeeta Shrestha

Thanks Antonio !! Super appreciate it :) .