Antonio di stasi

3 Questions 17 Answers 0 Followers

Questions related from Antonio di stasi

Primer for SFG RT PCR?

Does anyone have validated primers for QPCR on SFG retrovirus transduced cells? My goal is to calculate retro copy number in transduced cells, and for eventual titration of retroviral sup. For...

29 January 2020 7,708 0 View

How do you prepare your PCR for cloning?

How long do you digest your PCR? Do you believe cloning efficiency improves when isolating the band on a gel, rather then simply purifying it?

22 January 2015 7,985 7 View

Has anyone compared infusion vs. restriction enzyme based cloning?

Which one is more efficient? When can infusion be more indicated?

22 January 2015 2,571 3 View