Antonio di stasi

5 Questions 24 Answers 0 Followers

Questions related from Antonio di stasi

Primer for SFG RT PCR?

Does anyone have validated primers for QPCR on SFG retrovirus transduced cells? My goal is to calculate retro copy number in transduced cells, and for eventual titration of retroviral sup. For...

29 January 2020 7,720 0 View

Any advice on the NSG MICE TBI dose?

Hello, has anyone used 4+4Gy TBI for NSG mice irradiation and engraftment of: 1.Human leukemia cell lines 2.Human CD34 stem cells? Any less than that? 4Gy only? Thank you

02 December 2015 1,861 1 View

How do you prepare your PCR for cloning?

How long do you digest your PCR? Do you believe cloning efficiency improves when isolating the band on a gel, rather then simply purifying it?

22 January 2015 7,996 7 View

Has anyone compared infusion vs. restriction enzyme based cloning?

Which one is more efficient? When can infusion be more indicated?

22 January 2015 2,580 3 View

How do you administer TKI in relation to allogeneic HSCT?

When do you stop TKI before allo-HSCT? When do you restart them? At low dose and then escalate? When do you stop them?

22 January 2015 7,905 3 View