Vikas Gaur

2 Questions 6 Answers 0 Followers

Questions related from Vikas Gaur

Can we use miRNA cDNA, prepared using Taqman advanced miRNA cDNA synthesis kit with SYBR green assay?

I have prepared miRNA cDNA using Taqman advanced miRNA cDNA synthesis kit and used them with Taqman qPCR Assay. Now i want to assess expression level of more miRNAs in same samples using SYBR...

06 June 2019 8,896 13 View

How can i calculate fold change from ct values using delta delta ct method.?

I am doing taqman qRT PCR of miRNAs in leukemia patients to access specific miRNA expression pattern by using endogenous control. I got confused with fold change calculations using delta delta ct...

08 May 2018 6,437 5 View