How can I Calculate expression fold with CT values?

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Erdem Subak

this is a very helpful answer. Thank you Mr. Hall.

Zhiyong Zhang

Thank you Mr. Hall.

Pranav Vemula

Thank you a lot Mr. Hall. Your answer was very helpful.

Basma Ali

Laurence Stuart Dawkins-Hall

dear Dr Laurence i have seen your excel sheet ,i have two questions 1- if you have many untreated samples how would you calculate delta DELTA CT ?

why did yu calculate std and std error? thanks alot

Anshika Sharma

Hi there,

Thank you for your answer, I would like to ask how to proceed if the housekeeping gene Ct value is bigger than the GOI?

An Nguyen

Hi Laurence Stuart Dawkins-Hall

Can I use Livak method to compare the expression of 1 gene of interest (X) between 2 species of bacteria (group A streptococci vs group B streptococci)?

Y gene in both 2 species was use as refference gene (housekeeping gene).

Thank a lot

Priyobrata Sinha

The formula is 2^-delta delta Ct, where delta delta Ct=( delta Ct of treated - delta Ct of control). Delta Ct treated= (Ct of target gene in treated group - Ct of GAPDH or, beta actin or, 18s gene in treated group) and Delta Ct control= (Ct of target gene in control group - Ct of GAPDH or, beta actin or, 18s gene in control group). You can see the paper of Livak.

Thank you for your answer Laurence Stuart Dawkins-Hall

I have 2 more questions that need your help.

1. Which one should I use for presenting relative gene expression between control and treated group dCt, ddCt, 2^(-ddCt), log2 of fold change?

2. I found that some paper used "fold change" and some others used "relative expression of mRNA" in thier figures. Is there any difference between them?

please se my attached samples.

Bhagyashree Shelar

https://www.nature.com/articles/nprot.2008.73.pdf?proof=true

Please refer given link

Shashi Bhushan Chauhan

https://youtu.be/yahM5sRTlVM

@Erdem Subak