How can I Calculate expression fold with CT values?

More Erdem Subak's questions See All

How to learn historical or cultural subjects in the Montessori secondary programs?

I am looking for research that demonstrates practical and theoretical approaches. Thank you from now! #Montessori #Montessori Method

29 November 2020 3,339 6 View

Can you recommend simulations/games for statistics?

Can you recommend simulations/games for statistics as an in-class activity for undergrad students to teach them statistics, probability, distributions etc. as well as enjoying?

19 April 2018 1,492 5 View

How to assess a variable plays a role in a model as fixed or random effect?

01 March 2018 7,435 3 View

Is anyone familiar with silver destain?

I've stained my protein bands with Silver Kit (pierce). It includes a destaining reagent also, but the wash solution which conains NH4HCO3 is not provided. I couldn't find it in our lab. neither...

01 October 2014 369 3 View

How can I distinguish dead vs living PI staining for tsA201 cells?

I'm quite new in flow cytometry, especially the PI staining; what I did to see dead cells was following: after incubation with my antibodies and final spindown, I resuspended the cells in PBS and...

30 April 2014 9,931 4 View

Are there any mouse models for Behcet's Disease?

I want to study Behcet's Disease and I am searching for a BD mouse model. Is there any model about this?

28 January 2014 5,281 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

Basma Ali

Laurence Stuart Dawkins-Hall

dear Dr Laurence i have seen your excel sheet ,i have two questions 1- if you have many untreated samples how would you calculate delta DELTA CT ?

why did yu calculate std and std error? thanks alot

Anshika Sharma

Hi there,

Thank you for your answer, I would like to ask how to proceed if the housekeeping gene Ct value is bigger than the GOI?

An Nguyen

Hi Laurence Stuart Dawkins-Hall

Can I use Livak method to compare the expression of 1 gene of interest (X) between 2 species of bacteria (group A streptococci vs group B streptococci)?

Y gene in both 2 species was use as refference gene (housekeeping gene).

Thank a lot

Priyobrata Sinha

The formula is 2^-delta delta Ct, where delta delta Ct=( delta Ct of treated - delta Ct of control). Delta Ct treated= (Ct of target gene in treated group - Ct of GAPDH or, beta actin or, 18s gene in treated group) and Delta Ct control= (Ct of target gene in control group - Ct of GAPDH or, beta actin or, 18s gene in control group). You can see the paper of Livak.

Thank you for your answer Laurence Stuart Dawkins-Hall

I have 2 more questions that need your help.

1. Which one should I use for presenting relative gene expression between control and treated group dCt, ddCt, 2^(-ddCt), log2 of fold change?

2. I found that some paper used "fold change" and some others used "relative expression of mRNA" in thier figures. Is there any difference between them?

please se my attached samples.