Basma Ali

15 Questions 26 Answers 0 Followers

Questions related from Basma Ali

Which platforms helped you find a Phd in neuroscience/ oncology?

which websites that usually professors post phD vacancies on ?

28 November 2023 1,495 3 View

Any summer programs available to have hands on experience in patch clamp technique? theoretical course?

Any summer program to have hands on experience in patch clamp recordings ? also, any online theoretical course that you find beneficial ?

05 May 2023 8,362 5 View

Can Glucose absorption be decreased when co injected with oil?

Can intrapertonieal injection of oil decreases the absorption rate of glucose in rodents?

09 January 2021 4,592 0 View

Difference between Caliberator and Normalizer?

Just wanna make sure i understand right.

25 July 2020 5,366 4 View

Does oil injection affect strychnine absorption?

Can simultaneous injection of strychnine with corn oil decrease it's absorption ad effect?

19 July 2020 9,498 0 View

Is beta actin Rna stable?

in RTqPCR. if i have my target gene expression at ct 33 and beta actin at ct 17..i repeated and that's always the ratio..could my rna be degraded but i have good ct for beta actin how?

12 September 2019 3,494 3 View

Regarding Morris water maze test, if mice showed thigmotaxis in most of hidden platform training days whether reveciving LPS r control?

Should i exclude them from analysis? does LPS iP injection increases Thigmotaxis behavior?

25 March 2019 4,892 3 View

Hi i have a problem iam trying to make a new experiment study in qpcr to analyze all my plates by stepone machine ?

e but obviously i cant add the ALL PLATES as one of them was run at 10 mins holding time then after 7 mins the machine stopped due to emergency then i opened it again after a min and iu sat the...

19 January 2019 4,828 2 View

Do we always calculate Relative Quantification in Qpcr as 2 power - deltadeltact?

I am using stepone PLus machine and in excel sheet attached to my plate . i found effiency mentioned 1? what does this mean? iam quiet new to the device

06 January 2019 2,803 2 View

Nuclear protein PGC 1 alpha ? best way to extract from mouse brain ?

i have been struggeling to xtract it by ripa buffer ..then itake supernatent and heat in1 :1 ratio with 2x sample buffer at 95 degrees for 5 mins ..do yu have any suggestions ? it' s large ptn.so...

12 December 2018 2,532 2 View

Tips for large number of samples homogenization for rna extraction?

iam extracting RNA from brain tissues that are stored in -80 .should i get them all at same time from -80 which starting to homogenize some of them on ice or get few sames out of the -80 everynow...

23 November 2018 7,979 1 View

Can i multiplex 2 genes and singplex the rest in same run and compare results?

i mean i will do qpcr i will multiplex my housekeeping taqman gene (pl) vic dye with another taqman gene and will do the rest in singleplex format ? can i compare other genes expression to...

21 November 2018 8,855 3 View

If i add protein extract to sample buffer is there difference if i heated till 60 for 10 mins than at 95 for 5 mins?

10 mins at 60 degrees vs 5 min at 95 degrees

13 November 2018 6,841 6 View

I cant get protein signals using RIPA buffer for extraction of one brain hemisphere ? (myhous keeping ptn is cytoplasmic ptn beta actin ? no signal?

i doubt i have a problem in my extraction procedures .. i homogenize my brain in RIPA buffer NACl 150 mM , 25mM tris , SDS 0.1%, Sdium deoxycholate 1%, Triton x 1%, edta 5mM ... i use it in 1ul...

13 November 2018 7,504 5 View

I've measured my RNA sample conc. using Qubit and Using U.V double beam spectrophotometer .However cocentration was much higher i spectro than qubit?

Any help? which reading to trust ? if trusted the Spectro results i will load around 1ul or less from my Rna sample for reverse transcription but if i used qubit results i will load 20ul of Rna...

23 September 2018 7,485 6 View