Basma Ali

12 Questions 22 Answers 0 Followers

Questions related from Basma Ali

Any summer programs available to have hands on experience in patch clamp technique? theoretical course?

Any summer program to have hands on experience in patch clamp recordings ? also, any online theoretical course that you find beneficial ?

05 May 2023 8,348 5 View

Difference between Caliberator and Normalizer?

Just wanna make sure i understand right.

25 July 2020 5,342 4 View

Is beta actin Rna stable?

in RTqPCR. if i have my target gene expression at ct 33 and beta actin at ct 17..i repeated and that's always the ratio..could my rna be degraded but i have good ct for beta actin how?

12 September 2019 3,474 3 View

Regarding Morris water maze test, if mice showed thigmotaxis in most of hidden platform training days whether reveciving LPS r control?

Should i exclude them from analysis? does LPS iP injection increases Thigmotaxis behavior?

25 March 2019 4,872 3 View

Hi i have a problem iam trying to make a new experiment study in qpcr to analyze all my plates by stepone machine ?

e but obviously i cant add the ALL PLATES as one of them was run at 10 mins holding time then after 7 mins the machine stopped due to emergency then i opened it again after a min and iu sat the...

19 January 2019 4,807 2 View

Do we always calculate Relative Quantification in Qpcr as 2 power - deltadeltact?

I am using stepone PLus machine and in excel sheet attached to my plate . i found effiency mentioned 1? what does this mean? iam quiet new to the device

06 January 2019 2,780 2 View

Nuclear protein PGC 1 alpha ? best way to extract from mouse brain ?

i have been struggeling to xtract it by ripa buffer ..then itake supernatent and heat in1 :1 ratio with 2x sample buffer at 95 degrees for 5 mins ..do yu have any suggestions ? it' s large ptn.so...

12 December 2018 2,449 2 View

Tips for large number of samples homogenization for rna extraction?

iam extracting RNA from brain tissues that are stored in -80 .should i get them all at same time from -80 which starting to homogenize some of them on ice or get few sames out of the -80 everynow...

23 November 2018 7,958 1 View

Can i multiplex 2 genes and singplex the rest in same run and compare results?

i mean i will do qpcr i will multiplex my housekeeping taqman gene (pl) vic dye with another taqman gene and will do the rest in singleplex format ? can i compare other genes expression to...

21 November 2018 8,835 3 View

If i add protein extract to sample buffer is there difference if i heated till 60 for 10 mins than at 95 for 5 mins?

10 mins at 60 degrees vs 5 min at 95 degrees

13 November 2018 6,831 6 View

I cant get protein signals using RIPA buffer for extraction of one brain hemisphere ? (myhous keeping ptn is cytoplasmic ptn beta actin ? no signal?

i doubt i have a problem in my extraction procedures .. i homogenize my brain in RIPA buffer NACl 150 mM , 25mM tris , SDS 0.1%, Sdium deoxycholate 1%, Triton x 1%, edta 5mM ... i use it in 1ul...

13 November 2018 7,482 5 View

I've measured my RNA sample conc. using Qubit and Using U.V double beam spectrophotometer .However cocentration was much higher i spectro than qubit?

Any help? which reading to trust ? if trusted the Spectro results i will load around 1ul or less from my Rna sample for reverse transcription but if i used qubit results i will load 20ul of Rna...

23 September 2018 7,471 6 View