I have SEM images of the targeted particulate matter collected from the ambient air. How can I calculate the area of the identified single individuals to know better about the type of particulate and their respective size ?
The size of the particles in any powder sample is measured only by the radius of the particle and since we measure the average size, then we have to do statistics for all the particles in the sample in order to get good results. To get accurate results you have to use High resolution transmission electron microscope (HRTEM) for this investigation .
I think it is better to use BET technique for exact measuring the surface area of particles. But if it is not possible, you can use image analysis techniques by softwares such as CLEMEX and ImageJ.
Good Morning...
obtaining images of SEM is third dimension, I mean that would be suitable volume measure, if your interest is to measure areas ImageJ software is great, easy to use and very easy to understand ... There are a lot of publications referring this software
J Carlos Leon-Contreras
http://fiji.sc/Fiji
FIJI is an excellent pre-packaged distribution (described as batteries included) of image J which is bundled specifically for microscopy.
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