How can I break the bond between aminoacids and Gold Nanoparticles?

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Do I need to strip the PVDF membrane before staining it with colloidal gold?

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George Tsekenis

When you say amino acids, you mean any in specific or do you refer to proteins?

Fateme Salimian

No, They are free amino acids in Biological samples specially Methionine and Cysteine.

This earlier post that I have come across might be of help to you. Sonication or a reducing agent will usually do the job.

https://www.researchgate.net/post/How_can_one_break_a_gold-thiol_bond

Thanks Dr. George

Salimeh Ghorbani

your wellcome