Dear forum users,
I am trying to automate the generation of ROIs on a fluorescence time lapse image of SH-SY5Y cells to quantify potassium flux. We aim to calculate baseline response, fluorescence intensity, percentage fluorescence change, maximum fluorescence, and area under the curve. So far, I haven't been able to successfully use the ROI automation tool in NIS Elements AR as this does not accurately sense the cell boundaries. Is there another method using NIS elements, Image J, GraphPad, etc.? Thank you in advance!