Hello,
I want to attach a TMB dye to an antibody using cephalosporin-based linker that can be cleaved with B-lactamse enzyme?
This sounds interesting, I've never heard of this before. I'm not sure it will work because beta-lactamase cleavage of the beta-lactam ring does not result in conversion of one molecule into two. The ring just opens, at least initially. Subsequent electronic shifts may potentially lead to loss of some part of the molecule if it has the right substituents. However, the necessity of attaching the dye to one part of cephalosporin and a reactive group for linkage to the anibody to another part limit your options in this respect. In any case, what's the point of linking the dye to the antibody, then cleaving it off again?
im working on a diagnostic kit
i want to know the technique they used here : http://people.musc.edu/~beesonc/pages/research_cancer.htm
they attached Radionuclide to an antibody with cephalosporin-based linker
Hello Mohamed,
Have you found a response to your question because, i'm also interested by this kind of technique.
Thanks
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