How can I assess actual concentration of drug after subcutaneous injection into rodent’s hind paw during behavioral tests?

Viacheslav Viatchenko-Karpinski @Viacheslav_Viatchenko-Karpinski

12 December 2014 1 9K Report

I am faced with problem to use the same or close to the same actual concentrations of drugs in behavioral tests, as in electrophysiological experiments.

Does anybody know the ratio between the concentration of drug "in syringe" and actual concentration of this drug, that has an effect on nociceptive sensors in rodent's hind paw? A

How can I assess this actual concentration?

Amit Jain

If you have mean to measure the plasma concentration in that case measure it after 3 different concentratino dose given to animal. And evaluate which dose gives you plasma concentration close to your electrophysioly experiment and use that concentration in behaviou experiment.

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