DNA isolation from PAXgene Blood RNA tubes ?

I am in such unfortunate situation that I have to try to obtain whole-blood DNA from blood stored in PAXgene Blood RNA tubes [so in tubes containing some RNA-later'ish fluid]. Anyone tried to...

15 December 2020 9,445 2 View

Could the desmin mutation in myofibrillar myopathy be caused by an infection?

A patient with desminopathy (mutation Thr341Pro DES in a heterozygous state) with the progression of the disease has a decrease in taste and smell, immunosuppression, and an increase in IgA in the...

02 November 2020 5,886 6 View

How can we explain the decrease in taste in a patient with desminopathy (myofibrillar myopathy)?

A patient with hereditary desminopathy (mutation Thr341Pro DES in a heterozygous state) with disease progression has a significant decrease in taste. How can this fact be explained?

15 September 2020 3,742 4 View

Can Protein A tag interact directly with secondary antibodies on western blotting?

Hi, everyone! Does anybody have experience with WB of ProtA tagged proteins? Can it give a non-specific signal when you probe the membrane with control ab to different size protein?

29 November 2017 5,953 1 View

Can antibidy react with loading buffer?

Hi, I'm trying to perform some blotting with yeast Trf4 protein, but each time get strange result with nonspecific spots. It appear even in control wells, filled with water+loading dye, but not in...

26 June 2017 3,477 2 View

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05 June 2016 8,332 8 View

How can I make a whole-cell with hyperosmotic intrapipette solution?

I use regular KGluc solution, but with ajusted to 450 mOsm osmolarity. Bath solution is regular Tyrode-based, with normal osmolarity. Under these conditions gigaseal forms very quickly, but when I...

05 June 2015 6,674 4 View

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I am faced with problem to use the same or close to the same actual concentrations of drugs in behavioral tests, as in electrophysiological experiments. Does anybody know the ratio between the...

11 December 2014 9,153 1 View

Is it necessary to reduce the level of uric acid in the blood of a patient with desminopathy (myofibrillary myopathy)?

01 January 1970 422 3 View

How can I reduce the level of the pro-inflammatory cytokine interleukin-6 (Il-6) in myofibrillar myopathy?

Over the past six months, a patient with hereditary desminopathy (Thr341Pro DES mutation in the heterozygous state) has a 1.6-fold increase in the level of the pro-inflammatory cytokine...

01 January 1970 3,273 6 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

Compact antenna for the frequency range 1 MHz-5 MHz?

Hello everybody! I have a problem with developing a compact antenna for the 1MHz-5MHz frequency range. Tell me what type of antenna can be used and what books and articles to read to perform this...

27 February 2021 7,710 4 View

How to approach CFD simulation results of a flow past a cylinder?

Hi all, I'm doing a transient simulation of flow past a rectangular cylinder close to the free surface. As far as I'm concerned, the common practice is to discard the initial stages of the...

25 February 2021 1,348 3 View

Efficiency of Proximity Feed Patch Antenna ?

Greetings to all of You! I have searched some research papers for the designing of a proximity coupled patch antenna. Researchers are talking about the impedance bandwidth of the antenna but...

24 February 2021 7,662 3 View

How to visualise pathways from a phosphoproteomics dataset?

I am looking for something similar than Ingenuity Pathway Analysis (IPA) in which I can enter the proteins differentially phosphorylated and I can visualise the pathways these proteins are...

21 February 2021 8,923 4 View

How to phrase semi-structured interview questions using IPA without biasing superordinate themes?

Hello, I am preparing a semi-structured interview schedule for an interpretative phenomenological analysis study on marginalized individuals' career experiences as impacted by COVID-19. The aim...

19 February 2021 9,099 2 View

How to visualize stress in a coating defined by "Skin" in Abaqus?

Hello All! I'm trying to model a contact between a coated substrate and a tip in Abaqus. The coating is ultra-thin compared to the geometry of the substrate. So I defined the coating using the...

16 February 2021 1,594 3 View

How to perform Cox proportional hazard analysis or Kaplan-meier survival analysis in SPSS?

At first please see the attachment. This experiment was performed for a period of 7 days (n=100). After 7 days, we found different level of survival rate (%) for each treatment. Now, I want to...

15 February 2021 9,145 2 View

How to calculate visual angle of a visual stimulus?

I am currently trying to figure out how to calculate the visual angle of some visual stimuli I used in an experiment. I think I have all of the information I need to calculate this. One is a...

15 February 2021 3,189 2 View