Use serum of anyone having anti D in his/her blood (immunized during pregnancy or after mismatched transfusion). Incubate serum of this person with washed O Rh D pos red cells at 37oC for 10 min ( in ratio of 2:1 serum to cell). Wash cells and suspend them in CPD solution as 5 % suspension. Perform DAT on these cells which should be 2-3+. The cells will be good for 01 month. If want to extend life use cell stab of DiaMed or suspend them in Alsevers solution made in house.

Thank you very much Nuzhat!

Many years ago I used a chromium chloride coupling technique to produce monospecific IgG-, IgA- and IgM-coated cells as Coombs controls. I used serum from IgM Macroglobulinaemia, IgG and IgA myeloma patients to coat the cells. As Carlos says - some required diluting before use and needed testing with the other Coombs reagents for "non-specific" reactivity [i.e. testing IgG-coated cells with anti-IgA and -IgM. I seem to remember them lasting about three weeks, but that was ~30 years ago and the suspension media have improved a great deal since then. If I can find the method [no guarantee after all this time] would you like me to post it?

Thanks Graham! I won't be needing the technique. We decided to go for Gel Liss-Coombs technicque which does not require Coombs control.