Hossein Samiei Abianeh

5 Questions 4 Answers 0 Followers

Questions related from Hossein Samiei Abianeh

How can I activate D-biotin for conjugation to a recombinant protein?

I would like to biotinylate a recombinant protein via its primary amines. Can plain D-biotin be used directly, or does it need to be converted into a reactive form such as NHS-biotin? If...

last month 302 3 View

How can I validate my HRP-conjugated recombinant protein?

Hi colleagues, I recently used the periodate oxidation method to conjugate HRP (horseradish peroxidase) to a recombinant protein I expressed and purified. The goal is to use this conjugate as a...

22 April 2025 2,944 1 View

Why is the hydrodynamic diameter of my recombinant protein (~30 nm) larger than expected (2–10 nm) in DLS?

Hi everyone, I'm analyzing a recombinant protein (40 kDa) using Dynamic Light Scattering (DLS) and I'm getting a hydrodynamic diameter of ~30 nm in the number distribution, which is significantly...

22 April 2025 8,514 4 View

How to Obtain a PDB File for LPS for In Silico Docking?

Hello everyone, I am working on docking my recombinant protein with lipopolysaccharide (LPS) using in silico methods. However, I am having trouble finding a PDB file for LPS. I have checked the...

07 February 2025 5,204 2 View

Which is the best way to conjugation HRP to a recombinant protein?

Hello! I am working on conjugating horseradish peroxidase (HRP) to my recombinant protein for use in ELISA. I've searched for HRP conjugation methods but only found commercially available...

06 November 2024 6,893 0 View