Non motile black dots in animal cell culture, tightly adhered to the cells along the cell periphery. Is it a contamination? How to get rid of them?

I am seeing non-motile black dots of almost equal size along the periphery of skin melanoma cell line A375. They are present in almost all the cells. They don't turn the media turbid. However...

18 January 2021 5,151 1 View

Best flow cytometry markers for Thp1-derived M1 and M2 macrophages?

Hi everybody, I am trying to polarize and differentiate thp1 cells to M1 and M2 macrophages. There are lots of different protocols, so I've been trying different incubation times and...

02 November 2020 8,554 3 View

Have you ever seen two peaks after preparation small RNA library for NGS?

Hi, I have problem with my libraries, I use ClenTag Small RNA Library Preparation Kit by Trilink. The material is RNA from plasma cells. I have already done this two years ago and everythink...

25 August 2020 8,459 1 View

How to calculate the heat transfer coefficient value in a internal flow problem in Ansys fluent?

I'm simulating an internal flow of water to cool a hot rod. It's a transient simulation problem. I'm curious to find out the heat transfer coefficient from the simulation? Are any methods or...

10 August 2020 3,949 3 View

What are the key things to write and publish a paper in a good indexed journal?

The major spices and ingredients to add flavour in a paper for a journal in the domain of Computer Vision and Machine Learning.

27 June 2020 7,967 10 View

How to decide that a work is suitable for a conference or a journal in the field of Computer Science & Engineering?

What are the deciding factors to submit a paper in conference or journal.

21 June 2020 1,604 8 View

Whether Lambert Academic Publishing (LAP) provide printed copies to author? Do authors need paymet or sharing bank setails?

Hello Everyone !! Does anyone have any experience in publishing through Lambert Academic Publishing (LAP)? Recently I got a request mail from them regarding publication. I want to know about the...

25 April 2020 2,651 2 View

How can I write the .rpy and .jnl file in Abaqus cae for windows?

Hello :) I have the Abaqus CAE student version for Windows recently. My problem is: Abaqus does not write the .jnl and .rpy files automatically during a session as I am used to from the Linux...

30 March 2020 2,579 4 View

HSV1 in human tissue - dangerous after the fixation with PFA?

Hi, we are wondering about the HSV1 virus in human tissue. We want to perform in situ sequencing with this tissue, but we are not experienced with the virus-infected samples. Is it possible that...

14 October 2019 8,969 1 View

What details should be followed in MEGA-X for the Multiple Sequence Alignment of 16S data?

I have some 16S RNA sequence with which I want to do the phylogenetic analysis, so Initially I did BLAST against SILVA db and NACBI 16S db and went for MSA in MEGA-X using Muscle and Clustal both...

21 July 2019 9,320 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

Why am I getting 'Infinity' and 'NaN' in my mixed-mode energy release values?

Hi everyone, I am currently running a mixed-mode simulation of a DCB using a user-defined cohesive zone model (UEL), however, the results I am getting don't make sense. I used the same UEL for...

24 February 2021 4,157 1 View

Anyone give me assistance with analysing Gait Data?

I currently have collected Gyroscope/Accelerometer data (X/Y/Z) for my dissertation, but not very knowledgeable on how to process my data and apply Machine Learning? Can anybody help?!

24 February 2021 9,610 3 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View

Why does the sum of reaction forces not match the force component of a Free Body Cut in Abaqus?

I am using Abaqus to simulate a plate with in-plane loading. The boundary conditions allow the right edge to move up and down, the displacements on the left edge are fixed. There is no rotation...

23 February 2021 711 3 View

What low-cut filter (high Pass frequency) to be used for response spectrum (Tripartite plots) ?

What low-cut filter (high Pass frequency) to be used for the response spectrum (Tripartite plots)? I am currently working on differentiating between hard/soft site responses in Indo-Gangetic...

21 February 2021 9,273 2 View

The most suitable method to calculate the stress intensity factor in the presence of contact between the crack lips?

I'm trying to study cracked structures under a compressive load taking into account contact between the crack lips. I would like to know if the method of extrapolation by displacement remains...

21 February 2021 8,694 4 View

How can I obtain Reaction Forces in ABAQUS?

I am trying to simulate a simplified version of a beam resting on 10 points using Abaqus. The solutions I want to obtain are the Reaction Forces in each resting point or area when applying a...

21 February 2021 3,075 2 View

What cause the beating of this signal?

I am trying to simulate the impact of a sinusoidal force on a Beam, yet I registred this beating signal (Displacement by time). The freaquency of excitation was the same as the first natural...

19 February 2021 5,872 3 View