Please guid me, about acceptable delta ct values between target and reference gene.

07 July 2017 3,843 2 View

No title

04 July 2017 2,800 2 View

How can I make a stable & homogenous suspension of graphene in water?

I bought some graphene powder (1-3 layer) that was made by CVD. But I need to make a suspension of graphene in water to use it in an electrolyte solution. Can someone explain to me what the...

03 December 2013 527 19 View

Is it possible that the microRNA CT value is less than the internal control genes (ref. gene) in the patient's condition ?

in analyzing qPCR results, I saw the Ct value for one of my miRNAs is less than the two different selected ref. genes in the patient's condition, but not in control samples. Should I change my...

01 January 1970 2,649 2 View

How to get consensus of results produced by four miRNA target prediction algorithms (RNA22, miRanda, TargetFinder and psRNATarget)?

Different miRNA target prediction algorithms use different scores which is not comparable directly. So what is the most reliable way to get the consensus of results produced by different miRNA...

24 August 2020 9,659 4 View

Is it useful to have consensus of results produced by four miRNA target prediction algorithms (RNA22, miRanda, TargetFinder and psRNATarget)?

RNA22, miRanda, TargetFinder, and psRNATarget are four different algorithms aiming at finding miRNA targets. Many research-work claims at using a consensus of the result produced by these...

23 August 2020 5,058 5 View

Who knows a botanist in Mexico who could help me by collecting Mexican species of Gentiana?

Because of time limitation, I will not be able to visit Mexico in summer 2019. Yet, I would need a few species from there for some phylogenetic work by autumn 2019. These species are: G....

02 March 2019 3,997 2 View

Who knows a botanist in Mexico who could help me by collecting Mexican species of Gentiana?

Because of time limitation, I will not be able to visit Mexico in summer 2019. Yet, I would need a few species from there for some phylogenetic work by autumn 2019. These species are: G....

02 March 2019 9,199 2 View

How can i access the Miranda web page ?

unfortunately during this month i couldn't access to the miranda microRNA target prediction tool web page . http://www.microrna.org/ is the web page address was changed ?

05 June 2018 9,323 0 View

How to predict target genes of a mutated microRNA?

We obtained a de novo SNV on microRNA coding gene,and genomic data from mirbase told us that this SNV falls in sequence of mature microRNA.However,we failed to tell the altered sequence of this...

04 May 2018 2,079 2 View

Detecting miRNA target sites in lncRNAs?

Hello everyone, I am trying to predict miRNA target sites in long non-coding transcriptome. I have gone through the literature, but most of the algorithms such as TargetScan, miRanda, PicTar are...

16 April 2018 4,278 2 View

Detecting miRNA target sites in lncRNAs?

Hello everyone, I am trying to predict miRNA target sites in long non-coding transcriptome. I have gone through the literature, but most of the algorithms such as TargetScan, miRanda, PicTar are...

03 April 2018 9,937 3 View

Building SEMs in Lavaan (R) - How can I obtain better values of model fitness?

Hello ResearchGate community, I have been working on a series of SEMs as a part of my PhD. All of them seem to be running smoothly, but I am not being able to obtain decent measures of model...

03 April 2018 615 0 View

Is it necessary to use combine targets from 2 or more miRNA databases (e.g. miranda, targetscan, pictar) or just use only one database is better?

Hi! I am now working on miRNAs and I just new to this area. For the target prediction, since there are several databases and these are based on different algorithms. I just want to know would it...

31 December 2017 1,277 2 View