I require a DNA extraction kit or an easy method to extract DNA from Parus major blood samples.
Try DNeasy Blood an tissue kit... it extractes DNA from either blood or tissue... works well, and it's very fast and easy..
May I suggest using the prepGEM™ kit which I've used and achieved high-throughput DNA extraction excellent results. Ofcourse, that was what the kit have been formulated for, for animal tissue sample DNA extraction
See the article entitled "Comparison of preservation techniques for silkworm based on polymerase chain reaction products" may helpful to you
I don't know how you precipitated DNA by ethanol, if i will get answer i can clear the doubt
I usually use GenElute Mammalian kit by Sigma-Aldrich, I think it is a good way to perform DNA extraction from blood of bird stored in ethanol. Before to start, I suggest you to wash blood with NaCl 0.9% and remove supernatant. After this step, take 5-10 mg of pellet from the bottom of the tube and follow the standard protocol
I see this question is nearly 4 years old now - I would be interested in what method you ended up using?
I generally use a mag bead based approach for my DNA extraction. I prefer doing a gentle lysis. Mainly because I need high MW DNA for denovo genome sequencing. This is for genomes using long read sequencing technologies like PacBio. If it's Illumina sequencing, then the DNAeasy kit works just fine.
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