Best protocol for automated cell counting?

Hi all, I am performing an exosome quantification assay and I need to normalize my results to total number of cells. I will not be able to detach my cells from the plate, and therefore cannot use...

17 November 2020 6,583 2 View

Do I need collagenase to dissociate hepatocytes?

Hi all, after culturing hepatocytes in a monolayer on collagen in 24 well plates for 5 days, I attempted to trypsinize (0.25% trypsin) them to perform a cell count. However, none of my cells...

11 November 2020 2,478 2 View

Having set parameters correctly and edited sound files can anyone suggest why we are getting max pitch score above 300 using Praat?

We are using Praat to obtain min max and mean pitch from emerging adult narratives about relationship events. We have used the software for many years but lately noted more max scores above 300...

16 September 2020 1,961 4 View

Survey validation: more female that male respondents?

Hi all I'm currently working on a survey validation project. As a novice in this area, I have a question about female vs male respondents for the purpose of survey validation. Does it matter that...

13 September 2020 9,453 6 View

Can I use an IHC antibody for ICC?

I am planning a double antibody ICC on hepatocytes using polyclonal antibodies; however, one of the primary antibodies I want to use is only verified for use in IHC. Can I adapt it to be used in...

16 August 2020 4,073 3 View

What disinfectant should I use for cleaning the cell culture lab floor?

I've been using 1:10 to 1:100 bleach solution, but my PI has concerns of the corrosive nature/safety of bleach. I've been trying to find an SOP for cleaning lab floors; does anyone have...

10 March 2020 5,822 7 View

Meta analysis revman?

Hello all, I am trying to do meta analysis. I collected data from different studies (two arms studies, one arms studies, case report/series). I organized them into group/sub-groups of outcome I...

31 March 2019 5,705 5 View

Might be a silly question - how do you go from RPKM to a "percentage of expression"?

I received RNA seq data on a cell line and the level of expression of a certain receptor is "x"% (e.g. 29%). I'm not sure how this percentage was calculated from RPKM? Thnx in advance.

17 March 2019 4,402 3 View

How are Refractive index changes controlled?

When doing UV-Vis spectroscopy, how do you control for changes in refractive index? Isn't that something that the UV-Vis machine does automatically?

09 April 2018 8,581 4 View

Can anyone suggest a tool for assessing the attachment style in 12 to 18 year old adolescents?

I am proposing a research topic exploring adolescent sexual offenders and their attachment style and reslience.

26 February 2018 6,518 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

No title

01 March 2021 3,905 5 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

Does anyone have a sequence for the human CD34 promoter?

I'm looking for the human CD34 promoter sequence to engineer into a vector construct. Can anyone recommend a good source for this sequence? Thanks very much, Laura

25 February 2021 8,205 1 View

Is anyone working on a project focused on immigrants/newcomers working in the hospitality industry and how they have been impacted by COVID?

The on-going Covid-19 pandemic has had devastating economic effects on many industries. However, few have suffered to the same extent as full-service restaurants. Numerous studies have shown that...

24 February 2021 9,496 4 View

How do historians determine the credibility sources in modern history?

For a source to be considered reliable it must contain accurate historical information. But how does one determine the accuracy and reliability of sources in modern and recent history since not...

23 February 2021 1,346 13 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View

What is the role of work experience in motivating high tech entrepreneurship in Aviation industry?

Related work experience plays a vital role in motivating the Entrepreneurship. But when we talk about high tech entrepreneurship in Aviation industry, we talk about very high investment and high...

22 February 2021 376 5 View