How best to make DFO (obtained as solid from sigma) to treat cells for hypoxia studies? The question might be simple, but as I am starting out, I would like to know if one just weighs it in the tissue culture biological safety hood (BSLII), makes a 10X stock in PBS or media and then add it to the cell culture? It seems to decay with time, so how long is sufficient?
Sandra Zetterström Fernaeus
Why not make a stock solution e.g. 100x the final conc in water instead of PBS? Time of incubation should be minimum 2 hrs. The uptake is slow and believed to go through endocytosis. Its a relative large molecule.
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Binal Shah
Thank you for your answers. Can the left over be frozen? I know this sounds silly but sometimes stability/proper use details are hard to find and only experience helps.
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