Is there an open source tool the measures the GRAVY index of a peptide incorporating the PTMs and unusual amino acids (e.g. hydroxyproline)?

Right now, I am using ProtParam to compute GRAVY index of peptides. However, some of my peptides are post-translationally modified, and contains unusual amino acids. I assume that ProtParam does...

29 January 2021 8,300 4 View

When reporting mediation in a manuscript being submitted for publication, what have you found to be the easiest way to create your path diagram?

I am most familiar with using text boxes in WORD but I wasn't sure if that would be acceptable.

11 January 2021 3,585 1 View

How long does the peptide cleavage cocktail last before it gets expired?

The cleavage cocktail is composed of Phenol/thioanisole/water/TFA/triisopropylsilane. I only prepare 10 mL cocktail per peptide but can the activity of the cocktail prepared at 100 mL last for a week?

10 January 2021 7,424 3 View

What method is more useful for prepare metal/ metal oxide Nanocomposites?

If possible any easy preparation method

31 December 2020 1,662 3 View

Could you recommend an inexpensive camera for a prototype DIC system?

I was thinking of choosing Raspberry pi HQ 12MP. and what software do you recommend. I am working on a measurement system for a plate with an area of approx. 500x500 mm2

30 December 2020 3,064 2 View

Is there an interpretation for Lineweaver-Burk plot which linear regression lines intersect themselves on quadrant I?

Lineweaver-Burk replots for enzyme inhibition prints regression lines intersecting themselves on quadrant I. The interpretations for competitive, noncompetitive, or uncompetitive inhibition does...

24 November 2020 7,753 3 View

Protein long term storage can have an effect in pH changes?

I have a long term stability study with a protein that as an isoletric point lower than the buffer (Tris buffer), and I am seeing a sustantial drift from the initial pH to 12 month point....

10 November 2020 4,445 3 View

Advice on the culturing of EL4 lymphoma cell line?

Hi all, this is my first-time culturing lymphoma cell line. I obtained a frozen vial of EL4 (in the background of B6 mice) lymphoma cell line, and I am the only one culturing this line in the...

04 November 2020 932 3 View

When having a particular combination of two drugs in the same nanoparticle, how to discriminate between additive and synergistic anticancer activity?

Both additive and synergistic activities have been described for chemotherapy drug combinations co-loaded in the same nanoparticle. Such strategies have been aimed at cancer therapy via dual...

05 October 2020 5,027 5 View

How to find a partner university for an Academic conference?

I am organizing a conference for the first time and I would like to partner with an University for the conference. What are the things that I should do to invite/request a university. Whom should...

04 October 2020 7,530 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

No title

03 March 2021 5,360 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Mediation path analysis - Can I run two seperate models?

I am wanting to run a serial mediation model with 2 x IVs, 2 x MVs, and 3 DPs (all continuous). Is it problematic to run 2 different models for each IV, keeping the MVs and DPs the same in each...

27 February 2021 5,681 3 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View

Sample size determination for non-parametric tests?

How do I run a a priori sample size determination for non-parametric tests, like the Mann-Whitney U-test and the Kruskall-Wallis or other non-parametric tests that accord with the unpaired t-test...

24 February 2021 166 6 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View