Bacteria can change the pH of media by producing EPS. In my work, initial pH of nutrient broth is 6.5 and after the bacterial growth pH increases about 7.5. Which type of reactions can involved in media responsible for increasing pH?
Ashok Karan
Bacteria may evolved carbondioxide may convert to bicarbonate ions which may increase pH .
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Anwar Hussain
This might be due to conversion of protein into ammonia.
or Bacteria culture produces enzymes like protease, which has alkaline pH and microbe might produce products of alkaline nature.
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Salma Aktar
Better to specify the bacterial genera/name, btw, May be the bacteria is proteolytic, & thus, produce protease upon growth to degrade protein ino its component amino acids that have the ability to increase pH
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