Why is it that always the mutant protein is synthesized?

In autosomal dominant disorders I understand that the expression of one allele is required for the proper functioning. In case of mutations why not the protein from the normal allele get...

23 January 2015 5,309 5 View

What is the difference between physical distance and genetic distance ?

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16 April 2014 7,204 4 View

Why does the gel turn cloudy white as it gets solidified during a Native PAGE protocol?

see above

26 February 2014 8,606 8 View

Can somebody share their GC rich kit protocol for PCR amplfication of difficult templates?

see above

19 February 2014 1,054 5 View

Why are intron exon boundaries always AG, while exon introns boundaries are always GT?

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05 January 2014 307 7 View

Can somebody suggest a protocol for DNA extraction from amniotic fluid ?

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19 December 2013 8,349 3 View

What is basic, salt adjusted, and nearest neighbor when calculating the melting temperature for primer?

As given by some primer designing websites.

16 December 2013 2,106 7 View

Formula for calculating Tm of primers?

What are the different available formulae for calculating Tm of primers?

13 December 2013 4,932 12 View

No title

02 March 2021 3,060 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View