Are there well-established collaborative sorting tasks/datasets?

Hi, I am currently working on research on communication behavior in Mixed Reality. I am planning a study, where dyads perform a task, in which they have to collaboratively cluster or sort cards...

31 January 2021 4,979 3 View

Is it possible in smartpls to plot a confidence intervall in an interaction plot?

Hi everyone, I am using smartpls3 to analyze my model and I have a significant interaction in this model. I know the general function of plotting this interaction, but I am wondering if it is...

01 November 2020 8,753 2 View

Can someone give me an example for a questionnaire regarding roger's innovations in organizations?

Hello, I am looking for a questionnaire based on Roger's Diffusion of Innovations - Innovations in Organizations theory. Can someone give me an example?

15 September 2020 8,351 4 View

Is there a way to find out the number of articles, which appear, when I enter a specific term at the search?

for example, i was searching for "chronic neck pain and resistance Training" and so many publications appeared for that Topic, but i can't find the exact number of articles, which appeared here....

25 January 2020 9,565 2 View

How to separate two competing plasmids with same ORI?

I am currently working on the creation of an expression-Plasmid for Cas9 in E.coli. I have used the pET303-Vector as a backbone and inserted the Cas9-gene which I cut out of another Plasmid. My...

02 January 2020 4,634 3 View

Experiences with Twist Bioscience?

I am interested in your experiences and opinions on "Twist Bioscience". I want to order synthesized genes and they seem to have very competetive prices. Unfortunately they don't have any...

22 April 2019 8,047 2 View

FDA - Medical device with un/approve drug component?

Hello everyone, if a medical device that is already approved in the US should be modified with a drug component, what would be the difference in the FDA approval process, between an approved and...

14 March 2019 8,846 3 View

How to avoid wave reflection in Star-CCM+?

I am trying to simulate a VOF 5th-Order wave in Star-CCM+. The wave shall be 4.2 m long and 0.094 m high. For this I use a domain which is 5 wave lengths long. The two last wave lengths are used...

12 February 2019 7,980 16 View

What is the optimal input molarity for very short insert libraries on Hiseq 3000 / 4000?

A recent run of a very short insert library (average insert size 32bp) on a lane of Hiseq 3000 yielded only 50 million reads. Many clusters were excluded by the chastity filter due to mixed...

01 October 2017 9,535 1 View

What influences has UV-light on Niob(V)-oxid (Nb2O5)?

I wondered if Niob(V)-oxid as a dielectric high-index material is UV-sensitive like TiO2. Is there any fluorescence or decomposition? Any literature recommended?

13 February 2017 1,009 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View