Moritz Volland

4 Questions 6 Answers 0 Followers

Questions related from Moritz Volland

Is there a widely accepted or at least common abbreviation for RT-qPCR endogenous control (genes)?

I was wondering if there exists a widely used abbreviation for endogenous control (gene). Such as HKG and RG for Housekeeping and Reference Gene respectively. I have seen 'EC' used in McNeill et...

02 December 2015 6,490 3 View

How are BestKeeper (reference gene) standard deviation values calculated?

The BestKeeper (Pfaffl et al. 2004) Excel template is consistently giving me different (lower) standard deviation value for my "CPs" summary statistic (std dev (+/- CP)) than the one I calculate...

11 November 2015 7,234 0 View

What are the pitfalls of using 18S rRNA as an endogenous normalizer?

I am planning to test 18s rRNA among a number of putative housekeeping/reference/endogenous genes for real-time pcr for my cells. Now I read that "due to its extremely high expression in most cell...

15 July 2015 1,040 5 View

How do I create a protein-protein interaction networks for non-model organisms (marine bivalve)?

I have a set of proteins of interest from a non-model organism (the beautiful marine bivalve Ruditapes philippinarum) and I would like to "predict" / map possible protein-protein...

22 June 2015 9,868 3 View