Why does a Nested PCR protocol amplify samples and not amplify positive control?

I have this problem. Today I used a new positive control of an isolated NC-1 strain in cell culture and the problem persists. I performed a serial dilution test to test the sensitivity of the...

08 December 2020 6,252 2 View

How to avoid the formation of traces and dimers in DNA gel electrophoresis?

07 December 2020 3,052 3 View

Is the pipetting order of the positive and negative controls in the PCR important to avoid in a possible contamination?

I have been watching bands form on my negative controls. I have already changed all reagents, optimized the hygiene of materials and the environment and increased care in pipetting.

07 December 2020 9,090 3 View

Best methodology to calculate food footprint in a university campus through students inquiring?

Hello, Considering sustainability and environment negative impacts of animal based products, I would like to calculate food footprint in a Portuguese university campus through students...

05 December 2020 9,098 3 View

How to standardize and obtain a good PCR reaction with samples with a low and variable amount of DNA?

I work with the detection of N. caninum DNA in milk samples in milk samples. The DNA concentrations in my samples range between 2-90 ng / ul. I would like to establish and standardize a final...

01 December 2020 8,792 3 View

COVID-19, Human behaviour and wildlife impacts?

COVID-19 is causing a radical changes in Human behaviour around the world. Mandatory or voluntary isolation for months, suspension of the active management, etc.These changes will have positive...

24 March 2020 4,039 32 View

There are any component of gram + bacteria cell wall that can be used as a positive control?

Hello, I am going to evaluate the immunomodulatory effect of a gram positive probiotic bacteria in vitro and I need to use a positive control. I was thinking about LPS, but it's a component of...

11 March 2020 7,369 3 View

Can we use disposable gavage needles more than one time?

We use currently a steel gavage needle to deliver the substance to our rats. I once saw someone using a plastic needle, and I gave the idea for my advisor, using the plastic needle to lower stress...

02 March 2020 5,971 2 View

Is Innoscience a reputable research company?

Hi everyone! I received an invitation by email to join Innoscience as a collaborative consultant in Innoscience Research Sdn. Bhd (http://www.innoscience.org/). They claim to be an international...

20 January 2020 5,751 1 View

Dealing with next-gen SSR Microsatellite data from target enrichment experiment?

Hi All, we have a bioinformatics challenge and we would love any help this community can offer. We have data from a target-enrichment experiment that was supposed to capture certain...

09 January 2020 930 3 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Centrality measures computation in Biblioshiny/ Vosviewer?

26 February 2021 7,279 2 View

Add text at the beginning of a specific reference in Latex?

Hey researchers! The journal I'm submitting to wants datasets to be listed in the reference as [dataset] Authors; Year; Dataset title; Data repository ; Version; Persistent identifier (e.g....

18 November 2020 2,681 5 View

How do you include the literature in reference list that is not cited in the text?

Hi, as I know you should not cite the authors if you already include them in the sentence. But if I do not cite the study then it will not show in the reference list (I am using Zotero). How do I...

25 October 2020 9,203 3 View

How to import references to Endnote x9 from online databases and save text formatting?

I have problems with subscripts and superscripts in the titles. When I import references in .ris format directly from the Scopus, for example, text formatting is getting lost. So, I end up with a...

07 April 2020 713 3 View

Will the quality of the bibliographical references and consequently the richness of a scientific research depend on modernity or variability or other?

bibliographical references in thesis or paper or any scientific research

17 March 2020 2,618 3 View

How to cite Matlab ?

hello experts, do you know how to cite MATLAB(2019a) for academic research paper. i need to cite which version i have used for analysis but i didnt found any useful matter. i need to mention in...

11 March 2020 7,072 6 View

Can we use Ebird Personal Checklists as standard Journal References?

05 March 2020 2,002 7 View

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10 November 2019 9,644 1 View

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07 November 2019 2,121 1 View