Samples are to be homogenized in 9 volumes (9:1 w/w) of cold Tris-HCl buffer (0.1 M) at pH 7.4 to prepare 10% homogenate. Then, the centrifuge the homogenate at 500 g at 4°C for 10 minutes. The clear supernatant can be used for the assays

An easier way by homogization using PBS 1 ml/ 0.1 g tissue in a homgenizer