Manuela Buonanno

3 Questions 2 Answers 0 Followers

Questions related from Manuela Buonanno

Can the Andersen sampler be used to detect aerosolized MS2 on agar plates with E.coli on top?

Greetings, does anyone have experience using the Andersen 6-stage sampler to collect aerosolized MS2 bacteriophage? My idea is to place into the Andersen agar plates already with a layer of E.coli...

18 May 2024 5,793 3 View

Why soft agar on bottom agar layer split?

Greetings, I am using soft TSagar (0.75%) on a bottom 1%TSA to allow the growth of bacteria. But the day after I found that the agar split. Any suggestion as to why this happened and how to avoid...

31 August 2023 3,863 1 View

Hot to lyse a 3D human skin tissue model?

Hi, I need to lyse a 3D human skin tissue and use the tissue ''soup'' to do UV transmittance test. Which lysis buffer or disruption method would you advise (sonicator, freeze-thaw) 1) to keep the...

01 June 2021 4,697 2 View