Which open source tools are suitable for proteome analysis?

I am looking for an open source tool to analyse proteomics. I have used STRING database so far. I want to compare to different data sets (two different conditions) and visualize differences in the...

15 July 2020 2,252 7 View

Does anyone have any experience with collecting SES from young children (e.g. 8-9 year olds)?

In combination with school performance of 8-9 year old children we would like to survey the SES (socioeconomic status). Normally this is done through a parents' questionnaire. But does anyone have...

24 June 2020 4,566 1 View

Image recognition using SVMs vs. CNNs?

I'm new in CNNs. After discovering the pre-formed deep CNNs, which can be used for feature extraction, I ask myself if there is still an application area in the field of image recognition, in...

11 February 2019 2,691 1 View

Is there any study on the fat content influencing the saltiness perceived?

I have tried to use products with same salt content but having different levels of fat. The products are bread , butter and cheese.

26 March 2016 5,214 3 View

Is it possible to have from 1 sample total LDH and RNA?

11 January 2016 2,484 2 View

Does anybody know why the OD of the corticosterone ELISA is high although the concentration is supposedly very low?

We perfomed a corticosterone ELISA from ENZO and our data are misleading. The sample we analysed contain only low concentrations but we detected an OD that hypothesized high values. I think there...

15 October 2015 9,538 6 View

How can I analyze a DGGE gel with Bionumerics?

I want to analyze my gels with Bionumerics and I tried the same things as they explained in the videos and in the quick guide, but it does not work. When I try to create a new comparison, I do not...

13 February 2015 6,854 7 View

What is an ideal housekeeping to use for proteins expression?

I am investigating a process called epithelial-mesenchymal transition (EMT) in which epithelial cells lose their typical characteristic and acquire mesenchymal markers. Cells undergo EMT...

08 November 2014 8,257 7 View

Protein ubiquitination

What is the best way to identify if a protein is ubiquitinated?

05 September 2013 627 3 View

PCR to psa

I need to detect Pseudomonas syringae pv. actinidiae in symptomless plant material. What is the best PCR to do it, the PCR Rees-George et al. (2010) or Gallelli et al. (2011).I can only get one of...

21 May 2013 7,759 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

Can I use FLEXBAR to detect and remove barcodes out of my short reads without demultiplexing ?

Hello, I have demultiplexed my short reads and I have removed the Illumina adapter left in the sequences and now I need to remove the barcodes used by the sequencing centre from my reads. I have...

22 February 2021 9,873 3 View

Can you perform the Murexide test on samples fixed in Formalin?

I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples

22 February 2021 1,328 1 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View