Hi everyone. I am working on HT22 cell line in-vitro experiment to study dememtia.
I have mostly seen that they usually induce this cell death with H2O2 or glutamate.
BUT why very few people use SCOPOLAMINE?
Because in mouse or rat model, people usually use SCOPOLAMINE to make a dementia model.
Take this paper for example:
Animal experiment: use scopolamine indced modle.
Cell experiment: use glutamate and H2O2.
(Why didn't the author use scopolamine-induced HT22 cell death?)
You probably may ask the question to Pamela Maher, whos has published many papers about cell death using HT 22 cells. She is working at Salk institute in San Diego, California
you need to ask ourself, first, what are you going to study. Are you studying cell death per se? Do you want to study dementia, and if so, is this the right model and is cell death the most important factor to be studied? Cell lines are great to study biochemistry, sort of great for cell biology, much less great to study neuroscience, even less great to study neurodegeneration.