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17 February 2021 4,217 3 View

Which CGRP I could use to treat murine cells in vitro?

Does anyone worked with CGRP on mice. I m stuck with which the product good to use. I found Sigma products are good but Sigma has a lot of CGRP products and papers didnt mention the code or cat#...

16 February 2021 8,344 2 View

Why do cells die after adding MTT dye?

I was doing MTT assay, and in the last day before adding the MTT; the cells looked good (or so I think), but after aspiration of the drug and addition of MTT dye I noticed that most cells seemed...

07 February 2021 3,846 4 View

How do I get spectral power distribution (SPD) of my photoluminescence sample to calculate chromaticity coordinates?

Hi, I have photoluminescence samples which I already characterized and from that I already obtained the steady-state photoluminescence (SSPL) property. I want to draw CIE chromaticity diagram to...

24 January 2021 7,770 1 View

How can I stain 3d printed cells in hydrogel without damaging the gel?

I tried staining my cells in a gel using standard protocols with 4PF% for fixation and triton-X or saponin for permeabilization but the gel always gets damaged throughout the steps, and at the end...

17 January 2021 4,170 1 View

How can one synthesize the Inverted Core-Shell Particles (ICS)?

Dear RG colleagues, how can one synthesize the inverted core-shell particles (ICS) from a polymer and a Nano-metal oxide? Where the polymer is a core and the metal oxide is a shell. Under which...

01 December 2020 9,895 2 View

Why do I get low absorbance in MTT assay?

I have been trying to do MTT assay using HCT-116 cells but I have been getting very low absorbance readings (absorbance is usually 0.25 and below) even though iam seeding 30,000 cell/ well. I have...

16 November 2020 3,588 3 View

What do you think the percentage of educational lost by students in courses where labs and workshops are necessary?

During the current pandemic, most educational institutions all over the world were forced to on-line education which led students to be away from practice applications such as labs and workshops....

30 October 2020 3,254 16 View

What is the relationship (equation) between node transmission range and node density in wsn?

I am simulating routing protocol in wsn the orginal simulation was based on 802.11 mac and physical layer and I am using 802.15.4 as you know the transmission range in 802.15.4 is less, which...

20 August 2020 8,888 2 View

Are you interested in IEEE & Scopus Indexed Conference ?

Dear Colleagues, I am pleased to announce the 3ICT 2020 conference where you can publish your research finding without travelling. All papers registered and presented at 3ICT2020 conference will...

23 July 2020 4,077 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

How much should I centrifuge cells in agarose-coated plates to get spheroids?

Right now I am using HT29 cells, but I will also use HCT116 and probably breast cancer cell lines.

09 February 2021 2,063 6 View

C-4 I cervical cancer cell line tissue culture protocol?

Hi there! My lab has been brought an array of C-4 I cell line. The cells did not succeed in attaching to the bottom of the flask and remained floating in the media. Although we followed the...

04 February 2021 10,018 5 View