High Pure RNA Isolation Kit - isolation from RLT buffer? Is it possible?

Michal Zarobkiewicz @Michal-Zarobkiewicz

11 February 2020 1 664 Report

Does anyone know if it is possible to use Roche High Pure RNA Isolation Kit to isolate RNA from cells frozen in RLT (Qiagen RLT buffer + beta-merkaptoethanol)? As far as I am concerned, it seems that both RLT and Roche Lyse buffer are guanidine based. Therefore, I assume that it should work. Have anyone tried it before? Thank you!

Maral Ranjbar

Hi Michal,

You have probably reached an answer by now, but I also wanted to share my experience.

I had some sputum cells stored in Trizol (Thermofisher) and tried isolating RNA using Qiagen's RNAeasy kit. I got high yields of RNA from this kit. Therefore, I don't think using a different kit from your lysis buffer would be a problem as all these lysis buffers contain guanidine salt.

Michal Zarobkiewicz

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Science method

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