What is the Enrichr 'Sca1+ genes' list?

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The best RNA isolation kit for lncRNA RNA-Seq?

Hi, I am preparing a new project on lncRNAs in human cancer cell lines. I scanned through many high-impacted journals to find the best RNA isolation kit that would also isolate small RNAs and that...

09 February 2021 4,180 5 View

What tools or software can I use in order to visualize EEG brain to brain synchrony?

I want to visualize brain to brain synchrony results (using EEG) as an illustration of two 3D heads with lines connecting the electrodes positions between the two heads where brain to brain...

16 November 2020 7,742 2 View

Can anyone please post a link to a reliable rat cDNA primer database?

I am looking for a source of cDNA primers, rat genome, real time PCR. Previously I used rtprimers bd, but is seems to be out of service for a while now.

01 September 2020 5,132 3 View

Silica gel in the sample on RP-HPLC ?

Hi Guys! Excuse my ignorance but I have little experience with RP-phases. I've managed to initially purify my peptide product using normal phase flash-chromatography. Unfortunately, Ihad to use a...

20 July 2020 7,274 14 View

Anyone knows what causes eye lid problem in Siberian hamster?

Several months ago we observed some strange changes around eyes of several individual Siberian hamsters (Phodopus sungorus) in our colony (outbred). I attach pictures. It looks like an...

17 July 2020 9,941 3 View

Is there any way to receive Hirsch index excluding self-citations from the Web of Science core collection?

The only thing I see is the Hirsch index value including all citations. Despite looking through the WoS tools I could not find any way to exclude it. However, I'm quite sure there must be some way...

15 June 2020 6,324 2 View

What is the scaling law for thermal damage to biomolecules/proteins from ultrashort heating?

It is well known that heating can denature proteins. However, what does happen to proteins in the case of short and ultrashort (microsecond or nanosecond scale) heating to extreme temperatures...

05 June 2020 6,650 1 View

Challenges in medical education in pandemic era?

These days many of academic teachers around the world have switched to e-teaching due to current epidemiological situation related to SARS-CoV-2. This is a great challenge to both students,...

31 March 2020 2,025 5 View

COVID-19 at psychiatric clinic?

In the current pandemic situation, it is not possible to completely avoid a psychiatric patient carrying COVID-19 or being directly symptomatic. In addition, medical priority is to help anyone....

19 March 2020 4,389 10 View

How to visualise cholesterol crystals inside tissue/cell?

Hi! I am searching for a method of visualisation/staining protocol for cholesterol crystals (not a free cholesterol, just crystalized) in cells as well as tissues? anyone has any experience??

04 March 2020 5,669 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View