What is the Enrichr 'Sca1+ genes' list?

More Michal Smahel's questions See All

Determination of the supercritical conditions of the mixture?

Can anyone recommend a company or institution (write contact) that would be able to measure supercritical conditions for a liquid mixture? email: [email protected]

13 February 2024 2,183 1 View

What is the media image of social welfare institutions? What research is being done on this topic?

good day, I am conducting research on media images of (public) social welfare and media reporting of social workers. Can I ask for links to articles and studies what the media image of social...

07 February 2024 8,244 1 View

Why am I unable to open saved session file in AutoDockTools 1.5.7?

While trying to import Autodock session (AutoDockTools 1.5.7) I am receiving an error (attached below). All files work during simulations, yet it is impossible to open saved session file. mol.2...

19 December 2023 9,877 1 View

Can Thermal Shift/Differential Scanning Fluorimetry be used on soluble aggregates?

I Have Recently I have begun a project on expression and purification of a cytoplasmic protein. When running analytical Superdex 200 the protein elutes in the void volume despite the monomer being...

14 November 2023 6,876 1 View

Insurance of medical experiments in Germany?

Does an entity conducting medical experiments in Germany have to be insured against civil liability? Such a need exists in Poland and I'd like to know whether the same applies to Germany. Thanks...

25 September 2023 4,308 0 View

The order of Authors in two volumes of Proceedings is wrong. How can it be corrected?

Proceeding of the 46th Conference of the International Group for the Psychology of Mathematics Education (Vol. 3, online) Proceeding of the 46th Conference of the International Group for the...

24 August 2023 2,439 1 View

Wilcoxon matched-pairs signed-rank test vs. Wilcoxon signed rank test - choosing right statistical test ?

Hello, I have a question regarding statistics and choosing statistical test. My research question is whether certain medial procedure affects concentrations of extracellular vesicles (EVs) in...

20 August 2023 2,217 7 View

Which statistical test should i use?

Hi, i'd like to ask for advice with my paper "Comparison of correlations of stock prices before and during the pandemic". I already have the correlation coefficients calculated and shown in a...

02 April 2023 5,793 7 View

Looking for a statistician to do a meta-analysis. Interested to join?

Hello Everyone, I'm looking for a statistician to collaborate under a meta-analysis manuscript. Interested to join our research team? Please let me know on priv: [email protected]

13 March 2023 9,242 9 View

How to best isolate macrophages from PBMC?

I need to isolate macrophages from PBMC for RNA seq. The macrophages will be isolated 5 hours after activation (with Zymosan,through TLR 2/6). What would be the best method? And if I isolate them...

01 March 2023 9,813 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View