Michal Zarobkiewicz

3 Questions 4 Answers 0 Followers

Questions related from Michal Zarobkiewicz

High Pure RNA Isolation Kit - isolation from RLT buffer? Is it possible?

Does anyone know if it is possible to use Roche High Pure RNA Isolation Kit to isolate RNA from cells frozen in RLT (Qiagen RLT buffer + beta-merkaptoethanol)? As far as I am concerned, it seems...

11 February 2020 664 1 View

BD FACS Aria II poor purity. How to fix?

Hello! I am doing a bit of sorting recently. It used to be okay - approximately 90% purity. Unfortunately, yesterday it was a kind of random. The total number of cells in collection tube was...

23 July 2019 2,366 1 View

Is limited dilution analysis (LDA) a relevant and trustworthy method?

I got used to cytometric analysis performed on fresh blood. Recently, I read an old article, where LDA was used. Authors stimulated samples with either rIL-2 or PHA and thereafter performed...

23 January 2018 6,044 3 View