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Questions related from Michal Zarobkiewicz
Does anyone know if it is possible to use Roche High Pure RNA Isolation Kit to isolate RNA from cells frozen in RLT (Qiagen RLT buffer + beta-merkaptoethanol)? As far as I am concerned, it seems...
11 February 2020 703 1 View
Hello! I am doing a bit of sorting recently. It used to be okay - approximately 90% purity. Unfortunately, yesterday it was a kind of random. The total number of cells in collection tube was...
23 July 2019 2,403 1 View
Hello, I would like to try the EconoTaq Plus MasterMix for the duplex RT-qPCR with TaqMan probes. We have AB 7300 RT System. The problem się that Lucigen provides only the protocol for simple...
20 July 2019 5,404 0 View
I got used to cytometric analysis performed on fresh blood. Recently, I read an old article, where LDA was used. Authors stimulated samples with either rIL-2 or PHA and thereafter performed...
23 January 2018 6,093 3 View