How can I determine which dye I'm dealing with?

I am currently analyzing the composition of an unknown solution. It is a blue dye solution, but there are an awful lot of blue dyes. I have already analyzed the UV-VIS spectrum, the wavelength is...

08 October 2020 4,651 4 View

Management Innovation and Bahavior economic?

Hello guys! I'm looking for studies that address behavioral economics in Management innovation. Especially regarding the decision-making process.

13 September 2020 8,687 3 View

How to overlay and align different scale raster?

I need to overlay two rasters and the pixels of they must be in the same position to each other. I need this to perform a ecological niche model using a package in R. For this end all images must...

20 August 2020 9,503 4 View

Does anyone know how to quantify C-S-H in cementitious materials using XRD?

10 August 2020 4,207 11 View

Where can I find public medical image datasets?

Histological, X-ray or Endoscopic images, for example.

13 February 2020 3,674 3 View

What is the best way to isolate viable TILs from mammary mouse tumor?

It's been a while I'm trying to isolate tumor-infiltrating lymphocytes from 4T1 mice tumor, but I haven't been successful so far. I already tried different tumor processing protocols, but all of...

21 October 2019 6,898 3 View

How we can solve the metals demand and its increasingly scarcity on earth within sustainable ways?

Hey every one! Indeed, we need to improve how we use our resources, mainly the metals, those are base materials for humans activities. Thus, this questions aimed to discuss about solutions to...

22 March 2019 9,032 3 View

Anyone know the scale of tolerance of skin cells in culture, to ethanol?

I am working with cell culture and using ethanolic extracts of plants, I would like to know what percentage of ethanol from which influences the viability of the cells.

24 January 2019 8,924 3 View

Is this Intracellular Staining Protocol correct?

I'm currently trying to evaluate B10 cells on spleen samples, but I was not capable of detecting IL-10 by flow cytometry at all. After isolating splenocytes I performed two protocols: A) 6 hours...

12 September 2018 4,587 3 View

Mammalian inducible protein expression systems: is it possible to titrate for different protein expression levels?

Hi everyone, I am looking for mammalian expression system that allows for titration dependent expression of proteins. Meaning: a system that allows me to control the level of protein expression...

16 April 2018 6,438 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Is RD-ES grow attached or in suspension or both?

We have a old RD-ES cell line which is grow attached, but we got another vial of RD-ES which is very easy to get detached and grow in sphere in the medium. Do you know which morphology is normal...

24 February 2021 5,183 1 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View

How can I get the coding gene sequences of Hela and U2OS cell lines? Is mRNA sequence of Hsp90 alpha tissue specific??

I am looking for hsp90 mRNA sequence in Hela and U2OS cell lines, I have tried through ncbi but always the output is hsp90 in homo sapiens not in the specific cell line. Kindly, could any one...

22 February 2021 1,853 3 View

Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View