Hi, i want to work in targeted cpp-mediated delivery of pDNA. but i do not know if DNA can stably reach to nucleus?

More Tahereh Ebrahimi's questions See All

Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

29 July 2024 4,570 0 View

Hi dear researchers, do you suggest the best and shortest path for chemical synthesis and green synthesis of magnetite(Fe3O4)?

Suggest the best and shortest route for the chemical synthesis and and green synthesis of magnetite(Fe3O4) The chemical synthesis should contain the least dangerous chemicals along with full...

21 July 2024 2,476 2 View

Strategies for Effective Gold Nugget Exploration in Victoria, Australia?

Hello fellow researchers, I am currently reviewing technical papers and research on gold nugget exploration in Victoria, Australia. While there is a wealth of information available in academic...

21 May 2024 9,417 2 View

Video annotation tool for action classification?

Hello everyone, I have a dataset of videos for action classification, where each video contains multiple actions. I need to annotate these videos with the name of each class and the start and end...

17 May 2024 5,293 2 View

How can we assess the local people's attitudes toward wildlife?

We have recently chosen a flagship species for a protected area in Iran. Before introducing the flagship species to the local people, we want to measure the impact of the introduction on the local...

03 March 2024 9,356 3 View

Is it possible to extract the Histones from the already isolated proteins by Np-40 Lysis buffer?

I need to do western blot against H3K9me3 and I tested it once from my Protein extracted by Np-40 lysis buffer, I had a very nice band but the problem is the band was not at the right size (15...

11 February 2024 5,692 0 View

Could you please introduce some reliable references about electrical properties and structure of semiconductors?

I would like to find a reliable reference that includes electronic properties such as CB & VB DoS, electron & hole mobilities, thermal velocities of semiconductors like ZnTe, CdTe. I am...

10 February 2024 7,803 4 View

Explain the effect of strength exercises on sex?

One of the aspects of the impact of strength sports on the strength of family relationships

24 November 2023 4,105 1 View

Why IRC calculations using Gaussian results in a flat total energy curve?

Hi all I am studying a cationic cluster containing one molecule of methanol and one molecule of halobenzene, I am investigating a possible intra-cluster reaction leading to the formation of...

29 October 2023 6,171 6 View

Can self-healing hydrogels be formed quickly due to the presence of Schiff-base bond during fragmentation?

Hello all, Regarding self-healing hydrogels, can only the covalent Schiff-base bond break and form quickly, or do other bonds give this self-healing property to the hydrogel?

28 October 2023 1,050 3 View

Where can I find the recent population of Indian cities (2020s) ?

Census data seems only to reach the year 2011, but there might be estimates somewhere that I cannot find? I am looking not only for the largest cities but also towns etc. Thanks!

10 August 2024 857 4 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

How to increase simulation box size?

We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...

08 August 2024 4,842 0 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View