Is it possible to have two luminescence bands of the same peak position but with different intensities in single spectrum??

Hi! If it is possible to have two luminescence bands of the same peak position in a spectrum, what is the reason behind formation of two separate bands instead of one? This phenomenon is related...

26 February 2021 4,963 5 View

I'm looking for ways of analysing discoure complition tasks and ways of devising them, any suggestions?

I am an MA student working on pragmatics, specifically DCTs (discourse completion tasks). do you happen to know any books or articles on how to write and analyze DCTs? or is there any source for...

28 July 2020 5,136 2 View

Do you know any solvents to remove insects from the sticky traps?

We have been using thinner to remove the insect specimens from sticky traps. However, we would like to use other solvents with no toxic effects.

15 March 2020 6,789 12 View

Does anybody have an Android ".csv" Malware Data-set?

17 December 2019 761 3 View

How do I can remove the cladding a hard-clad fiber with chemical method?

How do I can remove the cladding a hard-clad fiber with chemical method?

15 December 2019 8,888 3 View

I run GSSHA model in WMS software but the result was not reasonable what is the problem?

i do it with 32 mm precipitation

05 August 2019 8,187 2 View

Why a plasmid in same condition give different amount of supercoil?

Did sequences affected amount of supercoil? Regardless of extraction in low temperature other factors can be handle for earn more supercoil? I have several plasmid that some of them ( such as...

11 January 2019 6,882 3 View

What is the best method for fresh vegetable disinfection?

what is the best method for fresh vegetable washing, in order to remove pathogenic microorganisms and pesticides.

30 December 2018 8,370 3 View

How we can calculate frequency of 52 bar truss?

I'm using the data available in the literature, I calculated the frequency but the same results can not be obtained. I used lumped mass matrix for calculating Mass matrix. M=ro*A*L/2*[1 0 0 0 0 0...

27 February 2018 7,155 5 View

What has been the "highest Saffron yield" you have recorded in the first year of planting in your research?

I do know about highest recorded Saffron yield in different studies, but your private experience with some explanations is intended here.

04 November 2017 8,482 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View