Any updated protocol regarding extraction and using of secondary metabolites of entomopathogenic fungus
Hello, see attached info and below ink may be useful for you,
https://www.researchgate.net/publication/45093062_Secondary_Metabolites_from_Entomopathogenic_Hypocrealean_Fungi
Good luck!
If you are hoping to isolate from entomopathogenic fungus you can use 1% acetic acid in 50% ethanol for extraction and then you can continue same procedure of fractionation, and regrading HPLC column its better to use biphenyl column
Ayman Turk your answer is quite helpful for me but I am at very initial stage. Can you please help me in understanding this in more deep way .
yes for sure, contact me by Pm if you want
Hi, I have performed untargeted LC-MS using thermo QE plus machine. The teacher gave me an excel sheet with lot of compounds. Can anyone help me in understanding that data?
03 April 2019 1,005 1 View
I need help regarding column chromatography. Your help in this regard is really helpful for me. On which parameters we can select the length and width of column. What size of silica is good for...
01 February 2019 6,119 9 View
I am growing this specific strain on SDAY @ 25-27C, dark condition as suggested from most papers and websites found. However, first it was producing very good amount of spores but now this...
31 December 2018 8,437 0 View
I want to prepare different bio pesticides formulation, Now I want to know about dust type formulation, at what ratio shold I mix active and inert gradient ? And for oil despersion how much...
11 December 2018 7,652 5 View
Hi. I have counted the spores on hemocytometer, now I need formula so that i can actually calculate them. Any one have proper formula? I just want to check how many spores in 5 ml of ddwater.?
08 September 2018 3,169 1 View
I need proper methodology regarding data collection of spore hydrophobicity of entomopathogenic fungi. Thanks in advance.
07 August 2018 2,348 0 View
I have total 20 Strains of four different fungal species, Now i want to characterize all of them. On what parameters, I can characterize them? Your suggestion will be highly appreciated thanks in...
06 July 2018 2,140 7 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...
01 March 2021 1,133 1 View
Incompletely cross-linked pdms are difficult to remove.
01 March 2021 798 3 View
Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.
27 February 2021 9,356 3 View
What is the most suitable medium of ascomycetes fungi ??
26 February 2021 4,413 1 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View
Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...
25 February 2021 1,660 3 View
Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...
25 February 2021 3,612 3 View
Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...
24 February 2021 5,749 3 View
I am using the Folin-Ciocalteu method for analysis of total phenolic content in ripe strawberries. I've read several protocol that suggest a methanol/H2O/formic acid (80:19:1) extraction solution....
22 February 2021 6,560 3 View