Hi, I have a question regarding the isolation of membrane proteins from homogenized tissues using ultra-centrifugation. Anyone suggest me please???

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Adam B Shapiro

If you have an antibody that recognizes this protein, then you can perform a Western blot to determine the molecular weight of the protein in the tissue extract.

Sachin Sharma

Thank you Adam, That is what I am doing but the antibody is generated against the whole protein and detecting other fragments of the mature protein also that got cleaved during the proteolytic processing or glycosylation.

Amir Hossein Doustimotlagh

May be the following link is helpful for you;

https://www.unr.edu/proteomics/sample-preparation/2d-protocols/protocol-protein-fractions

Thanks a lot