Hi, I am looking for a viscosifying agent to make cancer cell suspensions and avoid sedimentation?

More Pierre Danhier's questions See All

Please, could you delete that page of Pangou ?

Dear Editor, I'm shocked that you publicized some papers that are fake here see https://www.researchgate.net/scientific-contributions/Serge-Valentin-Pangou-2003482514 See also the pdf attached...

26 March 2024 7,871 2 View

Do you know a free software for estimating fish-body size on stereoscopic footages ?

Stereo-video surveys of demersal fish assemblages are widely used. A field and video‐annotation guide for baited remote underwa... Stereoscopic footages are used to assess fish-body size. A...

22 January 2024 6,253 0 View

Please suggest any documents or studies that use an emissions multiplier product matrix (EMPM)?

The emissions multiplier product matrix (EMPM) is an extension of the multiplier product matrix (MPM) based on the input-output table that takes into account the CO2 emissions generated by...

15 January 2024 7,111 0 View

Which chemicals that could be added in a sample to increase the level of dissolved oxygen?

My sample's dissolved oxygen is decreasing dramatically.What can I do to keep it stable or increase it again?

14 January 2024 3,308 7 View

A good pedagogic wargame?

hello, can you advise me a good wargame for pedagogy and training in strategy and tactic. I am searching something a bit serious designed in research and open.

30 November 2023 9,208 3 View

Is someone here with knowledge in TRNSYS software ?

I want help on design the draw profile daily on domestic water (throughout the day) using TRNSYS. Please your help !!!

29 November 2023 1,165 0 View

ResearchGate ne me reconnait pas Pierre Mbang comme étant Pierre Mazono Mbang. Comment y remedier?

Dans cet article Engineering homologous platelet-rich plasma, platelet-rich p... , je suis co-auteur. Lorsque je tente de le poster sur Researchgate, mon nom s'affiche comme Pierre Mbang et un...

22 August 2023 5,455 2 View

Hello scientists, I have a question: For citation and referencing, which citation style that combines both superscripts and square brackets?

Hello scientists, I am writing a paper to be published. I need to carry out in-text citations and referencing. Therefore, my question is: which citation style that combines both superscripts and...

21 June 2023 2,773 2 View

Comment corriger une référence ? / How do I correct a reference ???

08 May 2023 6,045 2 View

Comment analyser des distributions asymétriques ?

Chers collègues, comment analysez-vous ce type de données ? C'est une distribution très asymétrique. J'ai fait 2 transformations pour diminuer l'influence des données extrêmes : Racine carrée...

05 May 2023 2,582 1 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Which Scopus Journal provides the most affordable fees?

"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....

10 August 2024 8,621 1 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Could you please suggest methods to compare free protein and immobolized protein binding properties?

I have an antibody binding generic protein and I need to compare its activity in a free and immobolized form. I understand that there are a number of methods to determine Kd value of a free...

05 August 2024 5,311 0 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View

Tariq Khan

sterile DMSO. or FBS or both can be used together. 1% dmso in 10% fbs in media. for 100mL , 1 mL dmso and 10mL FBS & 89mL rpmi 1640 or dmem.

Goodwin G. Jinesh

For how long? Histopaque, matrigel are good options for short and long term respectively.

Pierre Danhier

Hi just the time needed to perform measurments (about 30 minutes), It is key that the thickener does not act as a reducing agent/prooxidant. Thx