What is the best method to conduct a meta analysis?

Dear Colleagues: For those of you who have conducted a couple of meta analyses, do you agree with these steps? What is the best method of extracting the data from the chosen studies? Formulate...

26 October 2020 1,539 6 View

Point zero charge Activate carbon, influence of mass?

Hello, I am going to do PZC of some activated biochar/carbon. I thought of doing it by Potentiometric titration with varying pHs and using a salt like NaCl at 0.01M. Now I am wondering: does the...

20 October 2020 5,108 5 View

Is there a database indicating the carbon and nitrogen storage capacity of vascular plants (in Europe)?

Hi everyone, I search a database or a review listing the capacity of vascular plants to storage carbon, nitrogen or other important chemical elements. I work actually on a mountain area (Swiss...

19 October 2020 8,596 3 View

Why is my Tet on system constitutively active and not dox inducible ?

I am using a Tet on system to overexpress a LncRNA in mouse ES cells, based on a piggybac vector (pXlone). I performed transient and stable experiments. In both cases, the LncRNA is expressed even...

13 September 2020 8,303 1 View

With increasing water shortage are the drought resistant plants going to be a solution for food production?

In many areas in the world the lack of water parallels the desertification.Is the creation of new drought resistant plants going to solve the problem for the world nutrition or do we have to...

20 August 2020 8,561 7 View

Do anyone know a good software allowing interactive 3D visualization of density plot?

Hello, I would like to make a interactive 3D representation of some data point. We are studying position of some cell of interest and we extract from serial coronal section XY position of the...

15 June 2020 8,117 2 View

No title

14 June 2020 2,475 7 View

How to extract Pd from Pd(NH3)4Cl2 solution ? Which electrolysis parameters should I choose ?

Hello everybody, I have a solution of Pd(NH3)4Cl2 that I got by refining spent leaching solution of gold. I did many cycles of ammonia (dissolution)/ chlorhydric acid (precipitation) and I got...

23 April 2020 3,048 1 View

How to take into account the SCR when computing the average SCL ?

Hi, I am looking for a suitable method to take into account the non specific SCRs when computing the average SCL. Since non specific SCRs artificially distort the SCL, I want to "remove" them...

30 March 2020 1,625 2 View

Do you think we can trap lymphocytes to manage Lupus crisis?

do you think we can trap lymphocytes to manage Lupus crisis? Something like put a generator of a olecul which attract its and after take of the generator. By this way we could down lymphocyts...

16 January 2020 8,382 6 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View