i want to do functional analysis of a gene from soybean and i use the overexpression vector with 35s promoter and HisTaq is there any body who tell me that which analysis I have to do I don't know any think.
I will be very thankful to you.
i need this software in english version?
07 August 2016 4,266 2 View
hello i want to ask that which enzyme i best to chose for restriction digestion of insert (2Kb) and vector (pCAMBIA 3301) like i want to use NcoI and BstEII which are located in the position of...
07 August 2016 9,730 3 View
hello research gate. 1-how to study the over-expression of a gene ? 2-like in general, what things we should have to consider for studying the function of gene by over-expression? 3-which is a...
05 June 2016 5,425 1 View
I have one gene and I want to know the family of that gene and is that possible to know the function of gene by using any of twee
05 June 2016 3,078 2 View
hello every, i want to know that can we use soybean mosaic virus instead of agrobacterium tumefecaion or agrobacterium rizhogen for transformation and study the function of gene.?
05 June 2016 1,888 1 View
Hello, can i ask a question that can any one explain me the difference between nucleic acids and amino acids, in the sense of which one is lighter or heavier,? next is then which one is easier to...
05 June 2016 460 0 View
hello, can you please tell me if i use the pcr product as a template and do the pcr again so is there any mutation will occur in my 2nd pcr product but as i check the re pcr products by using the...
05 June 2016 1,053 5 View
how to design the primer from only the CDS sequence as i want to clone my gene into the expression vector?
05 June 2016 1,820 3 View
i just want to know the predicated function of my gene can i do this by using any software?
05 June 2016 2,299 0 View
Hello, can any body please tell me how to improve the concentration of PCR product?or how to increase the concentrations of gel purified/gel extracted product? my preference is to improve the...
05 June 2016 8,533 2 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...
07 August 2024 269 3 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View