15 Questions 5 Answers 0 Followers
Questions related from Fahim Abbas
i need this software in english version?
08 August 2016 4,250 2 View
hello i want to ask that which enzyme i best to chose for restriction digestion of insert (2Kb) and vector (pCAMBIA 3301) like i want to use NcoI and BstEII which are located in the position of...
08 August 2016 9,713 3 View
i just want to know the predicated function of my gene can i do this by using any software?
06 June 2016 2,282 0 View
hello research gate. 1-how to study the over-expression of a gene ? 2-like in general, what things we should have to consider for studying the function of gene by over-expression? 3-which is a...
06 June 2016 5,406 1 View
how to design the primer from only the CDS sequence as i want to clone my gene into the expression vector?
06 June 2016 1,795 3 View
Hello, can any body please tell me how to improve the concentration of PCR product?or how to increase the concentrations of gel purified/gel extracted product? my preference is to improve the...
06 June 2016 8,521 2 View
hello, can you please tell me if i use the pcr product as a template and do the pcr again so is there any mutation will occur in my 2nd pcr product but as i check the re pcr products by using the...
06 June 2016 1,037 5 View
I have one gene and I want to know the family of that gene and is that possible to know the function of gene by using any of twee
06 June 2016 3,058 2 View
hello every, i want to know that can we use soybean mosaic virus instead of agrobacterium tumefecaion or agrobacterium rizhogen for transformation and study the function of gene.?
06 June 2016 1,862 1 View
Hello, can i ask a question that can any one explain me the difference between nucleic acids and amino acids, in the sense of which one is lighter or heavier,? next is then which one is easier to...
06 June 2016 438 0 View
as i used a super high fidelity DNA polymerase to finish PCR and then extract my desired length from the gel ,now i want to use an other 2 × pcr taq Master mix to add A Tail to my desired length...
05 May 2016 3,319 3 View
Hi, can any body wants to tell me the protocol and procedure of doing colony PCR. and which enzyme i can use to do this colony pcr. as my gene length as approximately 2 kb.?
05 May 2016 8,252 1 View
i don`t know how to prepare the apmicillin so can one one please help me to do this?
05 May 2016 7,264 2 View
Doing work on gene cloning, i want to now that why we insert our target gene into the cloning vector like pMD 19 T Vector, why not /can we just insert our gene into the expression vector directly...
05 May 2016 2,242 4 View
i want to do functional analysis of a gene from soybean and i use the overexpression vector with 35s promoter and HisTaq is there any body who tell me that which analysis I have to do I don't...
01 January 1970 3,433 0 View
