Hello can any one please give the the BioXM software ?

i need this software in english version?

07 August 2016 4,204 2 View

Which restriction enzyme is best to use as i want to use pCAMBIA 3301 for expression analysis of a gene?

07 August 2016 9,672 3 View

Can any one please tell me the difference between nucleic acid and amino acids?

Hello, can i ask a question that can any one explain me the difference between nucleic acids and amino acids, in the sense of which one is lighter or heavier,? next is then which one is easier to...

05 June 2016 369 0 View

How To find the family of a gene in soybean?

I have one gene and I want to know the family of that gene and is that possible to know the function of gene by using any of twee

05 June 2016 3,019 2 View

Using pcr product and do the pcr again ?

hello, can you please tell me if i use the pcr product as a template and do the pcr again so is there any mutation will occur in my 2nd pcr product but as i check the re pcr products by using the...

05 June 2016 1,004 5 View

Soybean mosaic virus use for transformation?

05 June 2016 1,820 1 View

How to design the primer from the CDS sequence?

how to design the primer from only the CDS sequence as i want to clone my gene into the expression vector?

05 June 2016 1,751 3 View

Concentration of PCR product?

Hello, can any body please tell me how to improve the concentration of PCR product?or how to increase the concentrations of gel purified/gel extracted product? my preference is to improve the...

05 June 2016 8,484 2 View

Is there any way to check the function of candidate gene by using any software?

i just want to know the predicated function of my gene can i do this by using any software?

05 June 2016 2,240 0 View

Can we use taq polymeraze to add poly A tails after finishing the pcr with HIgh fidelity DNA polymerase ,?

04 May 2016 3,284 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

Which CpGs have a significant effect on gene expression?

It is known that methylated CpG around TSS decreases gene expression. However, It is difficult to know exactly which CpG has a greater impact on gene expression. Are there any databases known...

20 February 2021 5,634 3 View