i just want to know the predicated function of my gene can i do this by using any software?
i need this software in english version?
07 August 2016 4,266 2 View
hello i want to ask that which enzyme i best to chose for restriction digestion of insert (2Kb) and vector (pCAMBIA 3301) like i want to use NcoI and BstEII which are located in the position of...
07 August 2016 9,730 3 View
hello research gate. 1-how to study the over-expression of a gene ? 2-like in general, what things we should have to consider for studying the function of gene by over-expression? 3-which is a...
05 June 2016 5,425 1 View
I have one gene and I want to know the family of that gene and is that possible to know the function of gene by using any of twee
05 June 2016 3,078 2 View
hello every, i want to know that can we use soybean mosaic virus instead of agrobacterium tumefecaion or agrobacterium rizhogen for transformation and study the function of gene.?
05 June 2016 1,888 1 View
Hello, can i ask a question that can any one explain me the difference between nucleic acids and amino acids, in the sense of which one is lighter or heavier,? next is then which one is easier to...
05 June 2016 460 0 View
hello, can you please tell me if i use the pcr product as a template and do the pcr again so is there any mutation will occur in my 2nd pcr product but as i check the re pcr products by using the...
05 June 2016 1,053 5 View
how to design the primer from only the CDS sequence as i want to clone my gene into the expression vector?
05 June 2016 1,820 3 View
Hello, can any body please tell me how to improve the concentration of PCR product?or how to increase the concentrations of gel purified/gel extracted product? my preference is to improve the...
05 June 2016 8,533 2 View
as i used a super high fidelity DNA polymerase to finish PCR and then extract my desired length from the gel ,now i want to use an other 2 × pcr taq Master mix to add A Tail to my desired length...
04 May 2016 3,335 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...
28 July 2024 2,979 1 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View