What types of stationary phases and solvent conditions are used for protein purity analysis via thin layer chromatography??

I have a protein labeled with a fluorescent dye (fluorescein) and I am hoping to get a quick measure of purity using TLC after dialysis. TLC is quick and easy so I am hoping to start there. What...

15 November 2020 7,319 3 View

Some of the universities held the conferences based on virtual technique because of the pandemic?

What is the scientific sides to this conferences?

09 June 2020 8,047 1 View

Hello all, could any one help me to find the properties of parrafine wax that I need to model this type of wax in Abaqus?

If any one know how to model wax in Abaqus that's will be very helpful.

27 October 2018 8,165 2 View

How to calculate kWh in a PV solar concentrator?

Hi All, I got confused in finding the proper method to calculate the kWh(Energy) that can my solar concentrator produce in a certain location. I 've got the Peak Sun Hours (PSH) calculated which...

04 September 2018 2,270 2 View

What is the ROC curve analysis?

Currently, I am working on medical image processing. So What is the ROC curve analysiscan be use for the classifiers?

02 March 2018 2,082 4 View

What are major problems in medical image segmentation?

Currently, I am working on microscope image segmentation. Is the any recommended about major problems in medical image segmentation

26 November 2017 9,163 8 View

How can I model damage evolution in composite material under abaqus standard?

Hello everyone, I am modelling a CFRP under quasi-static load. My model consists of 8 plies, each ply has a thickness of 0.125mm and I am using a cohesive surface between the plies to find the...

13 July 2015 4,074 7 View

What is the epidemyology of small bowel cancer?

01 January 1970 2,772 1 View

What are the benefits of being a reviewer for a journal?

What are the benefits of being a reviewer for a journal? I had receiving many requests to review a manuscripts for the journals.

01 January 1970 4,875 6 View

The inhibitory effect of Artemisia with nanoparticles on some metal oxides on some bacteria?

I studied for some time the inhibitory effect of Artemisia with nanoparticles on some metal oxides on some bacteria but did not get any result. Can a colleague help you with some advice?

01 January 1970 6,790 5 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View