Hi for every one I need the primer for ABO rs495828 ( ARMS or allele specific PCR)?

More Mazin H. Ouda's questions See All

Could you help me to identifying this benthonic foraminiferal species?

this thin section sample which taken from Late Campanian - Maastrichtian age carbonate Formation Northern Iraq.

20 February 2024 9,023 1 View

Hi , could you help me in identifying this benthic Foraminiferal species ?

this thin section which taken from Late Campanian - Maastrichtian Carbonate Formation Northern Iraq.

20 February 2024 6,855 2 View

IS their any relationship between ocean depths and foraminifera's abundant in gualities and guantities ?

I believe that.

19 January 2024 1,835 0 View

What does the Senonian Epoch consist of ?

the final Cretaceous Epoch which is dated at 88.5 - 65 Ma . and comprises the Coniacian , Santonian , Campanian age.

15 January 2024 8,905 3 View

What is the meaning of geological time scale ?

is a system used by geologists to divide earths history into distinct intervals.

12 January 2024 8,614 2 View

What is the index fossil?

index fossils are fossils of preserved remains or traces of a once living organism that lived in the specific geological past time and used to heip identify and date the age of rock layers.

11 January 2024 4,285 1 View

What are the index foraminifera fossils used in ancient marine depths studies?

agglutinated fossil foraminifera buried deeply in sedimentary basins can be used to estimate thermal maturity which is a key factor for petroleum generation , and estimate old marine depths.

10 January 2024 1,531 3 View

What is the foraminifera fossils?

Foraminifera are single celled shells or tests they are abundant as fossils for the last 500 million years.

10 January 2024 835 0 View

Hello everybody Can you help me get the pdf of (JCPDS Card No. 001-0830) and (JCPDS Card No. 45-1488) ?

This is to know the orientation of the crystals in the peaks (hkl) in the (XRD) pattern.

04 January 2024 2,788 5 View

Why 2024 aluminum alloy joints not recommended in brazing process?

many researchers diced not to braze the 2024 alloy even if the source was flame or vacuum furnace.

03 December 2023 3,011 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

Dimensions of an MJ Research 96-well alpha module?

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30 July 2024 2,867 0 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

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Michael Halim Popular answer

Interesting question. Following the discussion.

Jesus Rolando Delgado-Balderas

Hi Mazin H. Ouda

You can look the next link with information about your variant.

https://www.thermofisher.com/order/genome-database/?pearUXVerSuffix=pearUX2&elcanoForm=true#!/genotyping/assays/genotyping_all/?keyword=rs495828

It's a predesigned TaqMan Assay.

Nibras Saleam Al-Ammar

You can search for the suitable primers for your PCR target by using Gene NCBI website.

Good Luck

NIbras

Michael Halim