Hi, does anyone know method of quantification IAA (indole 3 acetic acid) using HPLC ?

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Ahmed M. Sayed

The following article will help...

Moein Moosavi-Nezhad

Dear Wiem

Take a look at these papers

Article Analysis of picogram quantities of indole-3-acetic acid by h...

Article Determination of indole-3-acetic acid, tryptophan and other ...

Article Simultaneous determination of gibberellic acid, indole-3-ace...

I hope they will behelpful.

Best.

Tasmia Bashir

First, select a good quality C18 column (e.g. 4.6 x 250 x 5u) and run a gradient of acidified Water (e.g. 0.1 % acetic) vs MeOH at 1.00 ml/min. Use a DAD to scan and detect (max ~ 249 to 254nm) and aim for a K prime of around >3.

You can also adapt the following method too;

Article A simple method for simultaneous RP-HPLC determination of in...

Anna Kisiala

Precise HPLC-MS/MS method: http://www.plantphysiol.org/content/177/2/476

Less accurate but easier to run, especially as a preliminary test: colorimetric method using Salkowski reagent and spectrophotometer: Technical Report Analytical Protocol for determination of Indole 3 acetic aci...